**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** DISRUPTIONS TO THE HUMAN GUT MICROBIOTA (DYSBIOSIS) BY ENVIRONMENTAL INTRUSIONS (E.G., BAD DIETARY HABITS, USE OF ANTIBIOTICS, STRESS, AND AGING) NEGATIVELY IMPACT HEALTH AND WELL-BEING. ORAL ADMINISTRATION OF PROBIOTICS (LIVE MICROORGANISMS) CAN PROVIDE A CONVENIENT INTERVENTION TO RESTORE HEALTHY GUT MICROBIOTA FUNCTIONING. HOWEVER, PROBIOTICS ARE SENSITIVE TO ENVIRONMENTAL CONDITIONS AND HAVE LIMITED STABILITY DURING FOOD PROCESSING, STORAGE, AND TRANSIT THROUGH THE GASTROINTESTINAL TRACT. THIS LIMITS THE EFFECTIVENESS OFIMPROVING HUMAN HEALTH. THUS,THERE IS A CRITICAL NEED FOR AN INNOVATIVE ENCAPSULATION TECHNIQUE THAT WILL PROMOTE PROBIOTICS' STABILITY DURING FOOD PROCESSING WHILE ENABLING EFFECTIVE DELIVERY TO THE COLON. THE PROPOSED STUDY AIMS TO DEMONSTRATE THE FEASIBILITY OF DEVELOPING 3D FOOD PRINTING APPROACHES TO ENABLE STABLE DELIVERY OF VIABLE PROBIOTICS AND GENERATE BENEFICIAL, SHELF-STABLE PROBIOTICS-LADEN FOOD PRODUCTS TO ADDRESS GUT DYSBIOSIS AND ASSOCIATED HEALTH ISSUES.