** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** AN OPPORTUNITY EXISTS TO EXPAND THE RECIRCULATING AQUACULTURE SYSTEM (RAS) INDUSTRY IN THE U.S. TO INCREASE THE DOMESTIC PRODUCTION OF SEAFOOD, BUT IT IS IMPORTANT TO FURTHER DISCOVER AND IMPLEMENT INNOVATIVE ENGINEERING TECHNOLOGIES TO EASE THE PATHWAY TO SUCCESS BY LOWERING OPERATIONAL COSTS AND REDUCING IMPACTS ON THE ENVIRONMENT. THE PROPOSED NOVEL TECHNOLOGY, PARTIAL-NITRIFICATION/DENITRIFICATION AND ANAMMOX (PANDA), IS A SHORT-CUT IN THE NITROGEN CYCLE THAT IS ENABLED BY LEVERAGING ANAEROBIC OXIDATION. THE BENEFITS OF IMPLEMENTING PANDA IN RAS (RAS-PANDA) WILL BE REALIZED BY REDUCING ENVIRONMENTAL IMPACTS (DECREASING WATER DEMAND, EFFLUENT LOADING RATES OF POLLUTANTS, OXYGEN DEMAND, GREENHOUSE GAS EMISSIONS) AND DECREASING OPERATIONAL COSTS. THE PROPOSED WORK HEREIN IS DESIGNED TO IMPROVE OUR KNOWLEDGE AND UNDERSTANDING OF HOW A NOVEL ENGINEERING TECHNOLOGY (PANDA) CAN BE USED TO IMPROVE AN ANIMAL SYSTEM (RAS PRODUCTION OF FISH) USING SENSING/AUTOMATION. THREE AIMS WILL BE CONDUCTED HEREIN INCLUDING AIM 1 THE TEAM WILL DETERMINE AND ASSESS THE BENEFITS OF IMPLEMENTING PANDA IN RAS BY SIMULTANEOUSLY EVALUATING TRADITIONAL RAS (NITRIFICATION ONLY) VERSUS RAS-D (NITRIFICATION AND DENITRIFICATION) VERSUS RAS-PANDA, AIM 2 THE TEAM WILL CONDUCT TECHNICAL AND ECONOMIC ANALYSES (TEAS) TO PROJECT THE TECHNOLOGIES TO COMMERCIAL APPLICATIONS AND TO UNDERSTAND HOW SYSTEM TYPE AND FEED LOADING RATES IMPACT OPERATIONAL COSTS, AND AIM 3 THE TEAM WILL CONDUCT LIFE CYCLE ASSESSMENTS (LCAS) TO CHARACTERIZE HOW THE DIFFERENT SYSTEMS AND FISH FEED LOADING RATES IMPACT THE ENVIRONMENT AND SOCIETY. IMPLEMENTING PANDA IN RAS WILL PROVIDE A BOOST FOR THE AQUACULTURE INDUSTRY AND WILL INCREASE OUR DOMESTIC PRODUCTION OF SAFE, TRACEABLE, AND HIGH-QUALITY SEAFOOD.