**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** SALMONELLA ENTERICA SEROTYPE DUBLIN IS ADAPTED TO CATTLE AND CAN ALSO INFECT HUMANS. IT CAN PERSIST ON FARMS FOR MONTHS TO YEARS AND CAN BE EXTREMELY DIFFICULT TO TREAT DUE TO ITS MULTI-DRUG RESISTANCE. THIS STRAIN IS SLOWLY INCREASING IN PREVALENCE ACROSS THE UNITED STATES AND CAN RESULT INSEVERE ECONOMIC LOSS. HOWEVER, NO DATA ARE AVAILABLE REGARDING THE HERD PREVALENCE OF S. DUBLIN IN PENNSYLVANIA DAIRY HERDS, DESPITE THIS PATHOGEN BEING ISOLATED FROM SEVERAL HUMAN CASES IN PENNSYLVANIA SINCE 2014, AND MANY ANIMAL CASES IN THE PAST TWO YEARS.THIS PROJECT AIMS TO ADDRESS THE POTENTIALLY UNDERDIAGNOSED PROBLEMS CAUSED BY SALMONELLA DUBLIN TO THE PENNSYLVANIA DAIRY INDUSTRY. WE WILL SCREEN BULK TANK MILK SAMPLES FOR S. DUBLIN ANTIBODIES TO DETERMINE HOW WIDESPREAD IT IS. FOR POSITIVE HERDS, WE WILL WORK WITH FARMERS AND USE ADVANCED DETECTION METHODS TO ISOLATE THE BACTERIA FROM ANIMALS AND THE ENVIRONMENT IN A SEEK AND DESTROY APPROACH. WE WILL ALSO USE A STRUCTURED RISK ASSESSMENT TOOL TO EVALUATE FARMS AND IDENTIFY MAJOR POINTS OF TRANSMISSION TO CREATE A TAILORED MITIGATION APPROACH DESIGNED BASED ON RISK ASSESSMENT IN EACH HERD.WE WILL DEVELOP A SUITE OF EXTENSION RESOURCES TO INCREASE THE LEVEL OF KNOWLEDGE ABOUTSALMONELLADUBLIN, AND TO CHANGE ATTITUDES AND BEHAVIORS TOWARD THE PREVENTION, DETECTION, AND CONTROL OFSALMONELLAIN DAIRY HERDS. WE WILL LEVERAGE THE EXTENSIVE REACH OF PENN STATE EXTENSION TO PROVIDE EDUCATIONAL RESOURCES FOR PA DAIRY FARM OWNERS AND EMPLOYEES, AS WELL AS THE VETERINARIANS WHO WORK WITH THESE DAIRY FARMS. THESE RESOURCES AND ENGAGEMENT OPPORTUNITIES WILL INCREASE KNOWLEDGE AND POSITIVELY IMPACT ATTITUDES AND BEHAVIORS ABOUT PREVENTING AND CONTROLLINGS. DUBLIN IN DAIRY HERDS AND REDUCING THE RISK OF ZOONOTIC DISEASE, ULTIMATELY LEADING TO IMPROVED MANAGEMENT AND BIOSECURITY INS.DUBLIN POSITIVE HERDS IN PA.