**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** TURKEY REOVIRUSES (TRVS) CONTINUE TO CAUSE DISEASE IN TURKEYS WITH VARIED PATHOGENICITY AND TISSUE TROPISM. THE INCIDENCE OF HEPATITIS AND MORTALITY IN TURKEY POULTS AND LAMENESS IN OLDER TURKEYS IS CONTINUOUSLY INCREASING EVEN WITH THE USE OF AUTOGENOUS VACCINES IN BREEDER TURKEYS. THE CHANGE FROM CAUSING MILD ENTERIC DISEASE TO ECONOMICALLY IMPORTANT ARTHRITIS AND HEPATITIS HIGHLIGHTS THE NEED FOR IMPROVING DIAGNOSTIC AND CONTROL MEASURES THROUGH RESEARCH AND EXTENSION.THIS STUDY PROPOSES TO TRANSLATE WHOLE GENOME SEQUENCE DATA INTO EASY-TO-UNDERSTAND GENOTYPING CLASSIFICATION WITH PROPER NOMENCLATURE OF SEQUENCES. THE GENOTYPIC CLASSIFICATION IS IMPORTANT TO UNDERSTAND SUBSTANTIAL HETEROGENEITY IN THE SPATIAL DISTRIBUTION AND SPREAD OF DIFFERENT STRAINS, POTENTIAL TURNOVER IN DOMINANT STRAIN ACROSS TIME, AND SELECTION OF VACCINE STRAINS. NON-INVASIVE ENVIRONMENTAL SAMPLING WILL HELP IN EARLY DETECTION OF TRV INFECTION IN THE FARM AS WELL AS BE HELPFUL IN TRACKING THE SOURCE OF INFECTION.EXTENSION ACTIVITIES WILL HELP ENHANCE KNOWLEDGE LEVEL OF DISEASE DIAGNOSTICIANS, FIELD VETERINARIANS, VETERINARY DIAGNOSTIC LABORATORY STAFF AND TURKEY PRODUCERS ABOUT THE SEQUENCE-BASED ANALYSIS, PROPER NAMING, AND GENOTYPING OF TRVS. IMPLEMENTATION OF NON-INVASIVE SAMPLING AND DECONTAMINATION STRATEGIES WILL HELP IN DISEASE DIAGNOSIS, SURVEILLANCE, AND MANAGEMENT AND CONTROL. THE INFORMATION GENERATED FROM THIS STUDY WILL BE DELIVERED TO FIELD VETERINARIANS, SCIENTIFIC COMMUNITY, AND POULTRY PRODUCERS. POSITIVE OUTCOMES FROM THIS STUDY WILL HELP IN IMPROVING DIAGNOSTICS AND CONTROL MEASURES FOR TRV-ASSOCIATED DISEASES.