INFERTILITY IN THE HIGH-PRODUCING DAIRY COW DURING THE POST-CALVING INTERVAL POSES AN IMPORTANT ECONOMIC AND MANAGEMENTPROBLEM FOR THE DAIRY INDUSTRY. NUTRIENT-HEALTH STATUS OF THE DAIRY COW IS THOUGHT TO HAVE A ROLE IN THIS INFERTILITY, BUT THE TISSUE ANDCELL-SPECIFIC MECHANISMS BEHIND IT ARE LESS UNDERSTOOD. OUR OVERARCHING HYPOTHESIS IS THAT DYSREGULATED NUTRIENTMETABOLISM IMPAIRS OVARIAN FUNCTION IN THE DAIRY COW. HERE WE WILL EXAMINE HOW DYSREGULATED METABOLISM IN THE DAIRY COWIMMEDIATELY AFTER CALVING AFFECTS GLUCOSE METABOLISM IN THE OVARY, SPECIFICALLY FOCUSING ON A NUTRIENT-SENSING MECHANISM CALLEDO-LINKED BETA-N-ACETYLGLUCOSAMINYLATION (O-GLCNACYLATION). OUR GOALS ARE TO CHARACTERIZE O-GLCNACYLATION IN OVARIAN FOLLICLES,AND TO DETERMINE THE GROWTH AND FUNCTIONAL EFFECT(S) OF MANIPULATION OF O-GLCNACYLATION IN GRANULOSA CELLS OF THESE FOLLICLES.RESULTS WILL PROVIDE INSIGHT ABOUT THE PREVALENCE AND INFLUENCE OF O-GLCNACYLATION IN THE GRANULOSA CELLS OF OVARIAN FOLLICLES,WHICHCOULD IMPACT FERTILITY. MODERATE O-GLCNACYLATION IS ANTICIPATED IN THE GRANULOSA CELLS OF HEALTHY, VIABLE OVARIAN FOLLICLES.THE ANTICIPATED IMPACT OF THIS WORK IS GREATER UNDERSTANDING ABOUT O-GLCNACYLATION AS A FORM OF GLUCOSE METABOLISM ANDPROCESSING IN THE OVARY THAT MAY AFFECT THE FATE OF OVARIAN FOLLICLES AND FERTILITY IN THE DAIRY COW.