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OUTBREAKS CAUSED BY VIRULENT AEROMONAS HYDROPHILA (VAH) SINCE 2009 RESULTED IN SIGNIFICANT LOSSES OF CATFISH FROM U.S. AQUACULTURE. THIS DISEASE IS A VERY HIGH PRIORITY FOR THE CATFISH INDUSTRY; IT HAS HAD A SERIOUS IMPACT ON THE REGION OF WEST ALABAMA AND EAST MISSISSIPPI, AND IT HAS BECOME THE MOST COMMONLY DIAGNOSED DISEASE IN THE MISSISSIPPI STATE UNIVERSITY AND AUBURN UNIVERSITY FISH DIAGNOSTIC LABORATORIES SERVING THESE REGIONS. THERE ARE LIMITATIONS IN THE CURRENT THERAPEUTIC AND PREVENTATIVE STRATEGIES AGAINST VAH. THE PROBLEM IS EXACERBATED BY A. HYDROPHILA STRAINS RESISTANT TO THE FDA APPROVED ANTIMICROBIAL (TERRAMYCIN®). THUS, IN THIS RESEARCH I AIM TO DEVELOP A DISEASE PREVENTION METHOD. SPECIFICALLY, THIS PROJECT WILL FOCUS ON VACCINE DEVELOPMENT AND EFFICACY EVALUATION IN CHANNEL CATFISH. OUR PRELIMINARY DATA REVEALED THAT RECOMBINANT VAH SURFACE PROTEINS (FIM, FIMMRFG, ATPASE, TDR, AND OMPA1) ARE EFFECTIVE IN PROTECTING CATFISH AGAINST MAS. FURTHERMORE, LIVE ATTENUATED EDWARDSIELLA ICTALURI VACCINE STRAIN ESC-NDKL1 IS AN EFFECTIVE CARRIER FOR THESE ANTIGENS USING A RECOMBINANT EXPRESSION SYSTEM. THE CENTRAL HYPOTHESIS IS THAT EXPRESSION OF VAH SURFACE PROTEINS IN A LIVE ATTENUATED E. ICTALURI VACCINE STRAIN WILL PROVIDE SIGNIFICANT PROTECTION AGAINST BOTH MAS CAUSED BY VAH AND ESC CAUSED BY E. ICTALURI, AND THAT VAH STIMULATES SIGNIFICANT INFLAMMATION IN THE ADAPTIVE IMMUNE RESPONSE IN CATFISH. IN THIS PROJECT, I WILL COMPLETE TWO OBJECTIVES: 1) CONSTRUCTION AND EVALUATION OF A RECOMBINANT LIVE ATTENUATED VACCINE STRAIN AS A DUAL STRATEGY TO PREVENT MAS AND ESC. THE GOAL IS TO DEVELOP AND EVALUATE STABLE RECOMBINANT ESC-NDKL1 STRAINS EXPRESSING ONE, TWO, OR THREE VAH SURFACE ANTIGENS. 2) DETERMINE EFFECTS OF VAH STRAIN ML09-119 ON THE CATFISH IMMUNE RESPONSE. THE GOAL IS TO DETERMINE THE ROLE OF INNATE AND ADAPTIVE IMMUNE COMPONENTS IN THE CHANNEL CATFISH RESPONSE TO VAH. THIS RESEARCH WILL YIELD AN IMPORTANT TOOL AND INFORMATION TO HELP COMBAT TWO DISEASES THAT HEAVILY IMPACT THE CATFISH INDUSTRY.

$100,220FY2022National Institute of Food and AgricultureUSDA

Mississippi State University, Mississippi State MS

Investigators

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OUTBREAKS CAUSED BY VIRULENT AEROMONAS HYDROPHILA (VAH) SINCE 2009 RESULTED IN SIGNIFICANT LOSSES OF CATFISH FROM U.S. AQUACULTURE. THIS DISEASE IS A VERY HIGH PRIORITY FOR THE CATFISH INDUSTRY; IT HAS HAD A SERIOUS IMPACT ON THE REGION OF WEST ALABAMA AND EAST MISSISSIPPI, AND IT HAS BECOME THE MOST COMMONLY DIAGNOSED DISEASE IN THE MISSISSIPPI STATE UNIVERSITY AND AUBURN UNIVERSITY FISH DIAGNOSTIC LABORATORIES SERVING THESE REGIONS. THERE ARE LIMITATIONS IN THE CURRENT THERAPEUTIC AND PREVENTATIVE STRATEGIES AGAINST VAH. THE PROBLEM IS EXACERBATED BY A. HYDROPHILA STRAINS RESISTANT TO THE FDA APPROVED ANTIMICROBIAL (TERRAMYCIN®). THUS, IN THIS RESEARCH I AIM TO DEVELOP A DISEASE PREVENTION METHOD. SPECIFICALLY, THIS PROJECT WILL FOCUS ON VACCINE DEVELOPMENT AND EFFICACY EVALUATION IN CHANNEL CATFISH. OUR PRELIMINARY DATA REVEALED THAT RECOMBINANT VAH SURFACE PROTEINS (FIM, FIMMRFG, ATPASE, TDR, AND OMPA1) ARE EFFECTIVE IN PROTECTING CATFISH AGAINST MAS. FURTHERMORE, LIVE ATTENUATED EDWARDSIELLA ICTALURI VACCINE STRAIN ESC-NDKL1 IS AN EFFECTIVE CARRIER FOR THESE ANTIGENS USING A RECOMBINANT EXPRESSION SYSTEM. THE CENTRAL HYPOTHESIS IS THAT EXPRESSION OF VAH SURFACE PROTEINS IN A LIVE ATTENUATED E. ICTALURI VACCINE STRAIN WILL PROVIDE SIGNIFICANT PROTECTION AGAINST BOTH MAS CAUSED BY VAH AND ESC CAUSED BY E. ICTALURI, AND THAT VAH STIMULATES SIGNIFICANT INFLAMMATION IN THE ADAPTIVE IMMUNE RESPONSE IN CATFISH. IN THIS PROJECT, I WILL COMPLETE TWO OBJECTIVES: 1) CONSTRUCTION AND EVALUATION OF A RECOMBINANT LIVE ATTENUATED VACCINE STRAIN AS A DUAL STRATEGY TO PREVENT MAS AND ESC. THE GOAL IS TO DEVELOP AND EVALUATE STABLE RECOMBINANT ESC-NDKL1 STRAINS EXPRESSING ONE, TWO, OR THREE VAH SURFACE ANTIGENS. 2) DETERMINE EFFECTS OF VAH STRAIN ML09-119 ON THE CATFISH IMMUNE RESPONSE. THE GOAL IS TO DETERMINE THE ROLE OF INNATE AND ADAPTIVE IMMUNE COMPONENTS IN THE CHANNEL CATFISH RESPONSE TO VAH. THIS RESEARCH WILL YIELD AN IMPORTANT TOOL AND INFORMATION TO HELP COMBAT TWO DISEASES THAT HEAVILY IMPACT THE CATFISH INDUSTRY. · GrantIndex