**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** MANY IMPORTANT AGRICULTURAL SPECIES ARE POLYPLOIDS, I.E., HAVE MULTIPLE COPIES OF THEIR GENOMES. THEY RANGE FROM STAPLE FOOD CROPS (POTATO, SWEETPOTATO) TO FRUITS (STRAWBERRY, KIWI, BLUEBERRY, BANANA), ORNAMENTAL FLOWERS (ROSES, CHRYSANTHEMUM), FORAGE CROPS, TURFGRASS, AND SUGAR AND ENERGY PRODUCTION CROPS (SUGARCANE). THE TRANSMISSION OF GENIC MATERIAL ACROSS GENERATIONS IN POLYPLOIDS IS MUCH MORE INTRICATE AND CHALLENGING TO UNRAVEL THAN DIPLOIDS, SUCH AS MAIZE, RICE, AND SOYBEANS. ALTHOUGH CHALLENGING, UNDERSTANDING INHERITANCE PATTERNS IS ESSENTIAL INFORMATION IN BREEDING PROGRAMS. WITH THE CORRECT ASSESSMENT OF THESE PATTERNS, IT IS POSSIBLE TO ASSOCIATE SPECIFIC GENOMIC POSITIONS TO IMPORTANT TRAITS OR EVEN FIND THE GENE RESPONSIBLE FOR THEM AND USE THIS INFORMATION IN BREEDING PROGRAMS.IN THE LAST FEW YEARS, WE HAVE DEVELOPED A SERIES OF COMPUTATIONAL TOOLS TO HELP BREEDERS AND GENETICISTS ANSWER THESE QUESTIONS BY ANALYZING GENOMIC DATA IN POLYPLOID SPECIES. WE DEVELOPED TOOLS SUCH AS VCF2SM AND SUPERMASSA FOR PROCESSING RAW DNA SEQUENCES AND IDENTIFYING GENETIC MARKERS, MAPPOLY FOR CONSTRUCTING GENETIC MAPS, AND QTLPOLY FOR LOCATING IMPORTANT GENES TO TRAIT PHENOTYPES ALSO, TO PERFORM PREDICTION. CURRENTLY, OUR TOOLS ARE IMPLEMENTED FOR LIMITED GENETIC DESIGN: FULL-SIB FAMILIES, AND WE ARE EXTENDING TO MULTIPLE FAMILIES. THIS PROJECT PROPOSES EXTENDING, EVEN MORE, OUR PREVIOUS POLYPLOID GENOMIC TOOL FOR GENERAL MULTIPLE-GENERATION PEDIGREE BREEDING POPULATIONS TYPICALLY PRESENT IN PRACTICAL POLYPLOID BREEDING PROGRAMS. MOREOVER, WE PROPOSE DEVELOPING A NEW DOWNSTREAM COMPUTATIONAL TOOL, CALLED DECISIONPOLY, USER-FRIENDLY AND OFFERS CLEARLY ILLUSTRATED ACTIONABLE INFORMATION TO ASSIST POLYPLOID BREEDERS IN MAKING SHORT- AND LONG-TERM BREEDING DECISIONS BASED ON THE COLLECTED AND LEARNED INFORMATION ABOUT THEIR BREEDING POPULATIONS DIFFERENT BREEDING OBJECTIVES.