**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** HONEY BEES ARE AFFLICTED WITH NUMEROUS PARASITES THAT THREATEN COLONY HEALTH AND POLLINATION SERVICES. ONE PARASITE WITH INCREASING IMPACT IS THE HONEY BEE-INFECTING TRYPANOSOMATID LOTMARIA PASSIM. THIS PARASITE IS FOUND ON SIX CONTINENTS (INCLUDING IN &GT;80% OF US MANAGED COLONIES) AND IS LINKED TO COLONY COLLAPSE IN BELGIUM, JAPAN, AND THE US. DESPITE ITS PREVALENCE AND EFFECTS, THE GENOMIC DIVERSITY AND TREATMENT OF THIS PARASITE HAVE RECEIVED SCANT ATTENTION, HINDERING ITS MONITORING AND CONTROL. OUR GOALS ARE TO ISOLATE AND CHARACTERIZE THE GENOMES OF L. PASSIM, USE THIS INFORMATION TO DETERMINE HOW THE PARASITE CAUSES DAMAGE, SCREEN FOR RESISTANCE ACROSS FIVE HONEY BEE LINEAGES, AND DEVELOP AND TEST IN FIELD COLONIES GENE-BASED CONTROL STRATEGIES USING RNA INTERFERENCE.