** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** AGRICULTURAL INSECT PESTS SHOW A REMARKABLE ABILITY TO EVOLVE RESISTANCE TO INSECTICIDES. ALTHOUGH INSECTICIDE RESISTANCE IS WIDELY CONSIDERED TO BE INEVITABLE, THE EVOLUTIONARY PROCESSES UNDERLYING THE EVOLUTION OF INSECTICIDE RESISTANCE REMAIN POORLY UNDERSTOOD. ONE POSSIBLE EXPLANATION IS THAT INSECTICIDE EXPOSURE MAY ALTER EPIGENETIC MODIFICATIONS, WHICH ALTER HERITABLE PATTERNS OF GENE EXPRESSION WITHOUT ACTUALLY CHANGING THE UNDERLYING DNA SEQUENCE. ENVIRONMENTAL STRESS HAS BEEN SHOWN TO INFLUENCE GENOME-WIDE PATTERNS OF DNA METHYLATION (A METHYL GROUP ADDED CPG DINUCLEOTIDE), ALTERING PATTERNS OF GENE EXPRESSION.THE COLORADO POTATO BEETLE (CPB), LEPTINOTARSA DECEMLINEATA, HAS BEEN EXTRAORDINARILY SUCCESSFUL AT ADAPTING TO ALL INSECTICIDES CLASSES, INCLUDING THE NEONICOTINOID INSECTICIDE IMIDACLOPRID. WE WILL USE AN EXPERIMENTAL EVOLUTION APPROACH TO TEST HOW BEETLE EXPOSURE TO SUBLETHAL DOSES OF IMIDACLOPRID CAN ALTER MULTIGENERATIONAL AND TRANSGENERATIONAL EPIGENETIC INHERITANCE OF INSECTICIDE TOLERANCE.