**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** GIVEN THE ECONOMIC VALUE ASSOCIATED WITH SEED STORAGE COMPOUNDS, SUCH AS FATTY ACIDS, PROTEIN, AND STARCH, AGRICULTURE-BREEDING RESEARCH HAS FOCUSED EXTENSIVELY ON IMPROVING TRAITS RELATED TO THESE COMPOUNDS. METABOLIC ENGINEERING STRATEGIES HAVE BEEN INSTRUMENTAL IN DEVELOPING MECHANISMS TO ENHANCE SEED COMPOUNDS SYNTHESIS IN CROPS. STILL, MUCH REMAINS TO BE LEARNED ABOUT THE COMPONENTS OF THESE REGULATORY NETWORKS TO PREDICTABLY MODIFY GENE TARGETS TO ENHANCE CARBON FLUX INTO DESIRED SEED STORAGE COMPOUNDS WITHOUT IMPACTING CROP YIELD.CAMELINA SATIVA(CAMELINA), A CONVENTIONAL OILSEED CROP OF THE MUSTARD FAMILY, HAS A HISTORY OF COMMERCIAL PRODUCTION IN EUROPE, THE NORTH AMERICAN GREAT PLAINS, AND THE PACIFIC NORTHWEST. U.S. CAMELINA HAS BEEN MAINLY USED FOR VEGETABLE OIL-DERIVED BIOFUELS. HOWEVER, NICHE MARKETS EXIST FOR NUTRACEUTICAL OILS AND ANIMAL FEEDS. CAMELINA OIL IS USED AS A FEEDSTOCK FOR JET FUEL PRODUCTION. AMONG OILSEED CROPS, CAMELINA OFFERS THE MOST EXCELLENT OPPORTUNITY TO ENGINEER MULTIGENE TRAITS RAPIDLY. CAMELINA CAN BE GENETICALLY TRANSFORMED BY FLORAL VACUUM INFILTRATION WITHAGROBACTERIUM TUMEFACIENSHARBORING DESIRED TRANSGENES, A METHOD USED FOR RAPID RNAI-BASED EVALUATION OF GENE CANDIDATES. CAMELINA HAS BEEN USED AS A PLATFORM FOR COMPLEX METABOLIC ENGINEERING FOR NUMEROUS BIOPRODUCT TRAITS. THE PROPOSED WORK IS BUILT ON OUR ONGOING RESEARCH THAT WILL IDENTIFY CRITICAL ENZYMES/GENES THAT REGULATE PRIMARILY SEED OILS, WHICH ARE COMPRISED MAINLY OF TRIACYLGLYCEROLS (TAG), AND TRANSLATE THIS KNOWLEDGE FOR THE DEVELOPMENT OF SEEDS WITH IMPROVED OILSEEDS. FURTHER, THE PROPOSED RESEARCH WILL EXPLORE METABOLIC ENGINEERING STRATEGIES TO ENHANCE OIL YIELD IN CAMELINA FOR INDUSTRIAL APPLICATION.