**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** YOUNG PIGLETS ARE OFTEN BORN WITH REDUCED GROWTH POTENTIAL AND INCREASED SUSCEPTIBILITY TO DISEASE. TO CORRECT THIS PROBLEM, PIGLETS ARE GIVEN MEDICATIONS SUCH AS ANTIBIOTICS. WE NEED TO REDUCE ANTIBIOTICS USE IN ANIMALS TO REDUCE THE CHANCES THAT ANTIBIOTIC RESISTANT ORGANIMS ARE PASSED TO HUMANS. THREFORE, WE ARE PROPOSING TO DETERMINE THE USEFULNESS OF LIVE YEAST FEEDING TO SOWS WHEN THEY ARE PREGNANT SO THAT THEY PASS FACTORS THAT WILL STRENGTHEN THE IMMUNITY OF THE PIGLETS. WHEN PIGLETS FROM SOWS THAT ARE FED LIVE YEAST ARE BORN, IT IS OUR EXPECTATION THAT TREATMENT WITH ANTIBIOTICS OR OTHER MEDICATIONS WILL NO LONGERBE NECCESSARY AGAIN FOR THE PIGLETS.OUR OVERARCHING HYPOTHESIS IS THAT FEEDING OF LIVE YEAST TO SOWS WHEN THEY ARE PREGNANT WILL LEAD TO PASSAGE OF IMMUNE STRENGTHENING FACTORS TO THE PIGLETS THROUGH THE MILK AND COLOSTRUM AND THIS WILL LEAD TO HEALTHIER PIGLETS THAT GROW BETTER.SPECIFICOBJECTIVES:1.DETERMINE EFFECTS OF MATERNAL LY SUPPLEMENTATION ON MILK YIELD, NUTRIENT COMPOSITION, PROTEOME, LIPIDOME AND MICROBIOME AND INFLAMMATORY STATUS OF POLYMORPHONUCLEAR NEUTROPHILS (PMN) OF COLOSTRUM AND MILK SAMPLES.2. DETERMINE HOWMATERNAL LY SUPPLEMENTATION DURING GESTATION AND LACTATION AFFECTS PIGLET INTESTINAL FUNCTION.3.DETERMINE EFFECT OF LY ON IMMUNE RESPONSE TO LPS CHALLENGE, EFFECT ON GROWTH AND NUTRIENT DIGESTIBILITY IN PIGLETS.4. WE WILL COMBINE ALL THE DATA GENERATED TO DETERMINE HOW LIVE YEAST IS WORKING TO AFFECT PIGLET PERFORMANCE AND HEALTH.EXPECTED RESULTS:WE EXPECT TO GET NEW DATA THAT WILL SHOW PROOF THAT FEEDING LIVE YEAST TO SOWS WHEN THEY ARE PREGNANT LEADS TO HEALTHIER PIGLETS BEING BORN THAT GROW BETTER.ANTICIPATED IMPACT:WE EXPECT THAT INFORMATION TO BE GENERATED WILL LEAD TO PIG GROWERS FEEDING LIVE YEAST TO THEIR HOGS DURING GEATATION. WE ALSO EXPECT THIS TO LEAD TO REDUCED USE OF ANTIBIOTICS AND OTHER MEDICATIONS TO TREAT PIGLETS. WE EXPECT THAT PIGS THAT WERE EXPOSED TO LIVE YEAST WILL GROW FASTER TO MARKET. THIS WILL LEAD TOMORE INCOME TO HOG PRODUCERS WHO USE THE FEEDING METHODS TO BE DEVELOPED.