** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** OUR PROJECT WILL INFORM THE EFFECTIVENESS OF MITIGATION STRATEGIES IN REDUCING RISK TO HUMAN HEALTH IN AGRO-ECOSYSTEMS BY DEVELOPING AN ADAPTABLE FRAMEWORK, CAMRADES, CONNECTING ANTIMICROBIAL RESISTANCE, AGRICULTURAL DECISIONS, AND ENVIRONMENTAL SYSTEMS. WE WILL INTEGRATE PREDICTIVE MODELS OF AMR TRANSPORT AND ASSOCIATED RISK TO HUMAN HEALTH, AND IMPROVE STAKEHOLDER UNDERSTANDING OF AMR, POTENTIAL RISKS AND MITIGATION STRATEGIES.OBJECTIVE 1. CHARACTERIZE AND PREDICT TRANSPORT OF AMR TARGETS VIA SURFACE AND SUBSURFACE PATHWAYS IN AGRO-ECOSYSTEMS.OBJECTIVE 2. INTEGRATE RESULTS INTO A NOVEL ADAPTABLE FRAMEWORK (CAMRADES) TO ASSESS RISK ASSOCIATED WITH TRANSPORT OF AMR THROUGH AGRICULTURAL ENVIRONMENTS TO HUMANS AS A RESULT OF AGRICULTURAL PRACTICES.OBJECTIVE 3. IMPROVE KNOWLEDGE OF AMR-RELATED RISKS AND INSPIRE ADOPTION OF PRACTICES AMONG FOOD PRODUCERS TO COMBAT AMR-RELATED HEALTH AND FOOD SAFETY RISKS ASSOCIATED WITH AGRO-ECOSYSTEMS.OUR FRAMEWORK INTEGRATES DETECTION OF ANTIBIOTICS, AMR GENES AND INDICATOR ORGANISMS AND EXPOSURE PATHWAYS IN THE ENVIRONMENT WITH HYDROLOGIC AND WATER QUALITY MONITORING. A KEY OUTPUT OF THIS EFFORT WILL BE A PREDICTIVE MODEL OF ANTIBIOTIC AND AMR SOURCES, FATE, AND TRANSPORT, WHICH WILL BE INTEGRATED WITH A NOVEL RISK ASSESSMENT. FINALLY, TO MAXIMIZE REALIZED AMR RISK REDUCTION, COMBINATIONS OF MITIGATION STRATEGIES WILL BE INFORMED BY POTENTIAL EFFICACY SCENARIOS ENCOMPASSING BARN, MANURE, AND LAND MANAGEMENT STRATEGIES AND DISSEMINATED USING THE RECOGNIZED AND TRUSTED IAMRESPONSIBLETM PROJECT PLATFORM AS WELL AS LIVESTOCK EXTENSION PROGRAMS. OUR PROJECT WILL CUMULATE WITH AN AMR SUMMIT AND AMR REDUCTION STRATEGY, A SCIENCE AND TECHNOLOGY-BASED FRAMEWORK TO REDUCE AMR IN SHARED WATER RESOURCES. WE WILL WORK CLOSELY WITH STAKEHOLDERS TO PROVIDE OPTIMAL FLEXIBILITY TO MEET PRODUCER NEEDS.