FLORIDA IS THE LARGEST CITRUS PRODUCER IN THE U.S., BUT THE CITRUS INDUSTRY RELIES ON THE APPLICATION OF MEDICALLY IMPORTANT ANTIBIOTICS TO CONTROL CITRUS GREENING DISEASE, WHICH HAS REDUCED FLORIDA CITRUS PRODUCTION BY 70%. THE IMPACTS OF ANTIBIOTICS ON ANTIMICROBIAL RESISTANCE BACTERIA (ARB) AND PLANT HEALTH LACKS ATTENTION. WE HYPOTHESIZE THAT ANTIBIOTIC APPLICATIONS INCREASE FREQUENCIES OF ARB, COMPROMISE PLANT HEALTH, AND ALTER THE ROOT-ASSOCIATED MICROBIOME, LEADING TO LOSS OF IMPORTANT ECOLOGICAL BACTERIA WITH PLANT HEALTH FUNCTIONS. OVERALL PROJECT GOALS ARE TO DEVELOP FOUNDATIONAL KNOWLEDGE AND AN APPLIED SUSTAINABLE APPROACH TO MITIGATE ARB ASSOCIATED WITH FRESH PRODUCE, WHILE INCREASING PLANT HEALTH, AND TO EDUCATE CURRENT AND FUTURE CONSUMERS AND MICROBIOLOGISTS. OUR OBJECTIVES ARE TO: ASSESS THE IMPACT OF ANTIBIOTICS ON ARB FREQUENCIES, PLANT PHYSIOLOGY, METABOLITES, AND MICROBIOME OF CITRUS TREES; DETERMINE THE EFFECTS OF PROBIOTIC SUPPLEMENTATION ON PLANT HEALTH AND ARB; AND EDUCATE UNDERGRADUATE STUDENTS AND SECONDARY SCHOOL TEACHERS ON ANTIMICROBIAL RESISTANCE (AMR) ACROSS THE FOOD CHAIN AND STRATEGIES FOR MITIGATING AMR.