AGROBACTERIUM IS AN ECONOMICALLY IMPORTANT PATHOGEN THAT CAUSES MILLIONS OF DOLLARS OF DAMANGE TO US CROPS. THIS PATHOGEN IS ABLE TO INFECT A WIDE RANGE OF PLANTS AND ONCE A PLANT IS INFECTED, THE ONLY FORM OF CONTROL IS TO DESTROY THE INFECTED PLANTS. IN ADDITION, THERE IS CURRENTLY ONLY ONE COMMERCIALLY-AVAILABLE PREVENTIVE AND IT HAS LIMITED USES. THE GOAL OF THIS PROJECT IS TO DEVELOP NEW CONTROL PRODUCTS THAT ARE EFFICACIOUS IN CONTROLLING AGAINST GENETICALLY DIVERSE AGROBACTERIUM PATHOGENS. WE HAVE IDENTIED STRAINS OF BACTERIA FROM THE BACILLUS GROUP THAT EXHIBIT TOXIC ACTIVITIES AGAINST AGROBACTERIUM GROWN IN CULTURE. WE HAVE FURTHER DEMONSTRATED EXTRACTS FROM BACILLUS ARE TOXIC AGAINST AGROBACTERIUM AND PROTECT PLANTS FROM DISEASE. THE SPECIFIC GOALS OF THIS PROJECT ARE TO: 1) DETERMINE THE IDENTITY OF THE COMPOUNDS IN THE EXTRACT, 2) UNDERSTAND HOW THIS EXTRACT WORKS, 3) UNDERSTAND WHY AGROBACTERIUM IS SENSITIVE TO IT, 4) DETERMINE WHETHER GENETICALLY DIFFERENT LINEAGES OF AGROBACTERIUM ARE SENSITIVE TO THE EXTRACT, AND 5) DEVELOP A SPRAYABLE FORM OF THE EXTRACT THAT CAN BE USED BY FARMERS AND GROWERS.