THE OVERALL PROJECT GOAL IS TO DEVELOP METABOLIC STRATEGIES TO OVERPRODUCE THE PHYLLOQUINONE (VITAMIN K1) PATHWAY INTERMEDIATE 1,4-DIHYDROXYNAPHTHOIC ACID (DHNA), WHICH IS PRECURSOR TO HIGH-VALUE PLANT 1,4-NAPTHOQUINONES (1,4-NQS). PLANT 1,4-NQS ARE OF INTEREST FOR THEIR NOVEL HERBICIDAL MECHANISM(S) OF ACTION. UNDERSTANDING FLUX REGULATION THROUGH THE PHYLLOQUINONE PATHWAY WILL REVEAL STRATEGIES TO INCREASE DHNA FOR SYNTHESIZING HERBICIDAL 1,4-NQS LIKE JUGLONE. WE HYPOTHESIZE THAT THERE ARE TWO MAJOR BOTTLENECKS IN THE PHYLLOQUINONE PATHWAY LIMITING DHNA PRODUCTION. WE HAVE PROPOSED METABOLIC ENGINEERING STRATEGIES THAT WILL ALLOW PLANTS TO OVERCOME THESE BARRIERS.THESE STRATEGIES WILL BE INTRODUCED INTO CAMELINA SATIVA TO CREATE A NEW COVER CROP VARIETY THAT WILL SERVE AS PLATFORM FOR PRODUCING HERBICIDAL 1,4-NQS IN CORN-SOYBEAN ROTATIONS. INTRODUCING A NEW HERBICIDE REQUIRES DEVELOPING A CORRESPONDING RESISTANCE TRAIT FOR CASH CROPS, SO WE WILL ALSOTEST STRATEGIES TO MANIPULATE GLUTATHIONE METABOLISM, WHICH OUR PRELIMINARY DATA INDICATE WILL INCREASE RESISTANCE TO HERBICIDAL 1,4-NQS LIKE JUGLONE. WE ENVISION THAT MULCHING ENGINEERED CAMELINA BEFORE PLANTING A 1,4-NQ-RESISTANT CASH CROP WILL ALLOW THE SYNTHESIZED 1,4-NQ-BASED HERBICIDE TO BE LEACHED FROM THE RESIDUE INTO THE FIELD.