**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** WASTEWATER REUSE FOR IRRIGATION REDUCES DEMAND FROM SURFACE AND GROUND WATER SOURCES. HOWEVER, THIS INNOVATIVE STRATEGY INVOLVES RISKS FROM THE HIGHER LEVELS OF ORGANIC AND INORGANIC CONTAMINANTS IN RECYCLED WASTEWATER (RWW) COMPARED TO CONVENTIONAL WATER SOURCES. DURING STORM EVENTS, CONTAMINANT CONCENTRATIONS IN RWW FLUCTUATE BECAUSE SEWERS RECEIVE VARIABLE LOADS OF MUNICIPAL WASTEWATER AND STORMWATER. THIS PROJECT WILL CHARACTERIZE STORMWATER INFLUENCES ON CONTAMINANT LOADS IN RWW AND THE ASSOCIATED EFFECTS ON RWW IRRIGATED CROPS. IN OBJECTIVE 1, WE WILL ASSESS HOW RAIN EVENTS AFFECT THE ORGANIC CONTAMINANT, HEAVY METAL, AND NUTRIENT PROFILES OF RWW FROM TWO SITES WITH SIGNIFICANT INTENTIONAL AND UNINTENTIONAL WASTEWATER INFILTRATION. IN OBJECTIVE 2, WE WILL DETERMINE HOW RWW IRRIGATION WITH VARIABLE STORMWATER INFLUENCE AFFECTS ACCUMULATION OF CONTAMINANTS IN ZUCCHINI PLANTS AND PLANT HEALTH AND NUTRITIONAL VALUE. OBJECTIVES 1 AND 2 WILL FEATURE BOTH TARGETED AND NOVEL NON-TARGETED ANALYSIS METHODS TO PROVIDE EXTREMELY BROAD COVERAGE OF ORGANIC CONTAMINANT DETECTION. IN OBJECTIVE 3, WE WILL DEVELOP NOVEL PREDICTIVE TOOLS TO DETERMINE THE EFFECTS OF USING RWW WITH VARYING LEVELS OF STORMWATER INFLUENCE FOR CROP IRRIGATION. OUR STRATEGIES INCLUDE CHEMICAL FINGERPRINTING AND CONTAMINANT PARTITIONING MODELS. THIS PROJECT WILL ENABLE DEVELOPMENT OF RELIABLE RWW QUALITY BENCHMARKS FOR ASSESSING NON-TRADITIONAL WATER USE FOR IRRIGATION. FURTHERMORE, RESULTS WILL BE USED TO PROVIDE GUIDANCE TO FARMERS AND WASTEWATER UTILITIES ON BEST MANAGEMENT PRACTICES FOR IRRIGATION WITH WASTEWATER RECLAIMED DURING STORM EVENTS.