**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** DESPITE THE KNOWN TOXICITY OF MICROPLASTIC POLLUTION AND THE U.S. COMMITMENT TO PROTECTING WATER QUALITY AND REDUCING FRESHWATER DEMAND FOR IRRIGATION, THERE ARE NO CURRENT STEPS IN SEWAGE/WASTEWATER TREATMENT THAT REDUCING MICROPLASTIC CONTAMINATION. YET, DOING SO WOULD PERMIT INCREASED USE OF SUCH NONTRADITIONAL WATER SOURCES IN IRRIGATION OF AGRICULTURAL LAND. A NUMBER OF ENZYMES ARE CAPABLE OF BIOREMEDIATION OF MICROPLASTICS, THE BEST CHARACTERIZED OF WHICH IS POLYETHYLENE TEREPHTHALATE HYDROLASE, ISPETASE. THIS ENZYME HAS BEEN ENGINEERED TO INCREASE STABILITY AND ACTIVITY, YET PRODUCTION OF TRANSLATABLE ISPETASES HAS BEEN HINDERED DUE TO USE OF SMALL, MANUALLY SELECTED LIBRARIES, AND A FOCUS ON FREE ENZYME AS OPPOSED TO ATTACHING THEM TO SOLID SUPPORTS, A STRATEGY THAT OFFERS SPATIAL CONTROL, STABILITY, AND REUSABILITY BENEFITS. WE PROPOSE TO INTEGRATE COMPUTATIONAL AND EXPERIMENTAL TOOLS, ALONG WITH REAL-WORLD APPLICATION STUDIES TO ENGINEER ISPETASE THAT PERMIT BIOREMEDIATION OF WASTEWATER TO REDUCE ITS CHEMICAL CONTAMINATION BY MICROPLASTICS. ENGINEERING AFFINITY TAGS TO PERMIT SITE-DIRECTED BINDING ONTO SILICA PARTICLES WILL PERMIT THEIR CO-LOCALIZATION IN LAGOONS ALONGSIDE TARGET MICROPLASTICS. THE LONGTERM IMPACT OF THIS RESEARCH IS THE DEVELOPMENT OF AN ENZYME BASED BIOREMEDIATION SYSTEM THAT REDUCES THE FRESHWATER DEMAND FOR IRRIGATION BY SUBSTITUTING THE USE OF NONTRADITIONAL WATER SOURCES WHILE RETAINING APPROPRIATE SOIL HEALTH, IN SUPPORT OF USDA GOALS FOR IMPROVING AGRICULTURAL SUSTAINABILITY.