DEVELOPMENT OF EFFECTIVE TREATMENT AND REUSE OPTIONS FOR NONTRADITIONAL WATER SOURCES IS A CRITICAL STRATEGY TO HELP INCREASE FOOD PRODUCTION WHILE MINIMIZING FRESHWATER USE FOR CROP IRRIGATION. THE GOAL OF THE PROPOSED RESEARCH PROJECT IS TO UNDERSTAND THE POTENTIAL RISK OF CROP IRRIGATION WITH TREATED UNCONVENTIONAL OIL AND GAS (UOG) PRODUCED WATER ON SOIL AND CROP HEALTH USING A COMBINATION OF WATER TREATMENT TECHNOLOGIES, TOXICITY TESTING, AND WHEAT IRRIGATION TRAILS. SPECIFIC OBJECTIVES:INVESTIGATE THE TREATMENT EFFICACY OF DIFFERENT TREATMENT TECHNOLOGIES FOR UOG PRODUCED WATER INTENDED FOR AGRICULTURAL IRRIGATION.CHARACTERIZE THE TOXICITY AND CHEMICAL COMPOSITION OF UOG PRODUCED WATER BEFORE AND AFTER TREATMENT.IDENTIFY THE IMPACT OF TREATED UOG PRODUCED WATER ON SOIL AND CROP HEALTH USING GREENHOUSE-BASED CROP IRRIGATION STUDIES.ELUCIDATE THE INFLUENCE OF IRRIGATION WATER QUALITY ON THE PLANT METABOLOME FOR IDENTIFICATION OF ADVERSE CROP EFFECTS.THIS PROJECT WILL HELP GUIDE SELECTION OF NONTRADITIONAL WATER SOURCES FOR IRRIGATION BY ENSURING THAT THE TREATED WATER CAN SUSTAIN CROP YIELDS WHILE PREVENTING ADVERSE EFFECTS SUCH AS SOIL SALINITY, COMPROMISED PLANT IMMUNE SYSTEM AND METABOLISM, AND THREATS TO WATER AND FOOD SECURITY.