**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** CATTLE DRINKING WATER POLLUTED BY PER- AND POLY-FLUOROALKYL SUBSTANCES (PFAS) HAS LED TO CONTAMINATED MILK THAT, IF CONSUMED BY HUMANS, WILL CAUSE SEVERE HEALTH ISSUES. THE LONG-TERM GOAL OF THIS PROJECT IS TO ESTABLISH A GREEN, NONTHERMAL PLASMA-BASED TECHNOLOGY FOR ON-FARM WATER TREATMENT THAT WILL BE COMPACT, EASY TO OPERATE, AND EFFICIENT IN REMOVING TOXIC PFAS IN CATTLE DRINKING WATER IN ORDER TO PREVENT MILK CONTAMINATION. THIS ADVANCED TECHNIQUE IS BUILT ON A NOVEL, CONTINUOUS-FLOW, LIQUID-PHASE PLASMA DISCHARGE (CFLPPD) REACTOR THAT IS HYPOTHESIZED TO EFFECTIVELY AND EFFICIENTLY DECOMPOSE PFAS WHILE PRESERVING WATER QUALITY ATTRIBUTES. SPECIFIC OBJECTIVES OF THIS PROPOSAL ARE TO 1) STUDY THE TECHNICAL FEASIBILITY AND MECHANISM OF EFFECTIVE REMEDIATION OF TYPICAL PFAS SUBSTANCES (PFOA AND PFOS) BY THE IMPROVED CONTINUOUS FLOW LIQUID-PHASE PLASMA DISCHARGE (CFLPPD) PROCESS DESIGNED FOR ON-FARM USE; 2) IDENTIFY SIGNIFICANT FACTORS THAT INFLUENCE THE PFAS DEGRADATION EFFICIENCY AND WATER QUALITY, AND THE BEST OPERATIONAL MODE BY THE CFLPPD PROCESS. SUCCESSFUL COMPLETION OF THIS SEED PROJECT WILL LAY THE TECHNICAL GROUNDWORK FOR REVOLUTIONIZING THE ON-FARM WATER TREATMENT SYSTEM TO TACKLE PFAS CONTAMINATION AT REDUCED COST AND REDUCED ENERGY USAGE. THIS WORK ADDRESSES THE PROGRAM AREA PRIORITIES OF NIFA FOUNDATIONAL AND APPLIED SCIENCE PROGRAM A1521, I.E., ENABLE ENGINEERING SYSTEMS FOR FORESTRY AND NATURAL RESOURCES; PLANT AND ANIMAL PRODUCTION.