**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** ELEVATED LEVELS OF RESISTANT BACTERIA AND RESISTANCE GENES ARE FINDING THEIR WAY INTO THEENVIRONMENT THROUGH THE LAND APPLICATION OF MANURE ASSOCIATED WITH ANIMAL PRODUCTION FACILITIES.WE PROPOSE TO COMBAT AMR IN THESE SYSTEMS BY: DEVELOPING IMPROVED TOOLS TO BETTER INDICATEAMR RISK IN AGROECOSYSTEMS, IDENTIFYING THE BEST PRACTICES FOR MITIGATING AMR DURING MANURESTORAGE AND TREATMENT, AND PROVIDING A BETTER UNDERSTANDING OF HOW AMR DEVELOPS DURING SWINEMANURE MANAGEMENT. OUR RESULTS WILL BE DIRECTLY COMMUNICATED TO PRODUCERS AND STAKEHOLDERSTHROUGH OUR LONG-STANDING MANURE MANAGEMENT EXTENSION PROGRAMS. WE WILL CHARACTERIZE AMR RESPONSE TO MANURES UNDER VARYINGANTIBIOTIC USAGE AND PRESSURES. FURTHER, WE WILL EVALUATE SEVERAL MITIGATIVE TECHNOLOGIES FOR THE EFFECTIVENESS AGAINST AMR.THE PROPOSED RESEARCH WILL UNIQUELY BRING TOGETHER BOTH TRADITIONAL AND NOVEL AMR INDICATORS TOFILL IMPORTANT KNOWLEDGE GAPS ASSOCIATED WITH THEIR LINKAGES TO AMR EMERGENCE AND PERSISTENCE.THE DEVELOPMENT OF THESE TOOLS ARE FRAMED THROUGH EXPERIMENTS THAT ADDRESS OPEN QUESTIONSABOUT THE MOST EFFECTIVE STRATEGIES TO DECREASE AMR DURING MANURE MANAGEMENT.