**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** ANTIBIOTIC RESISTANCE HAS BEEN WIDELY IDENTIFIED AS A GLOBAL HEALTH ISSUE OF CRITICAL FUTURE SIGNIFICANCE. HOWEVER, QUESTIONS REMAIN OVER THE SPREAD OF ANTIBIOTIC RESISTANCE IN AGRICULTURAL SYSTEMS WHERE ANTIBIOTICS AND ANTIBIOTIC RESISTANCE DETERMINANDS (ARDS), E.G., ANTIBIOTIC RESISTANCE GENES (ARGS), ANTIBIOTIC RESISTANT BACTERIA (ARB), AND MOBILE GENETIC ELEMENTS (MGES), CAN BE APPLIED TO SOILS IN WASTEWATER AND MANURE.USING A SERIES OF GREENHOUSE AND OUTDOOR (LARGER-SCALE) EXPERIMENTS, WE WILL QUANTIFY THE MOVEMENT OF ANTIBIOTICS AND ARDSBETWEEN WASTEWATER/MANURE, SOIL, PLANTS, AND ANIMALS (EARTHWORMS). UNDERSTANDING THESE PATHWAYS IS OF CRITICAL IMPORTANCE IN ASSESSING THE POTENTIAL FOR ANTIMICROBIAL RESISTANCE TO SPREAD THROUGH FOOD CHAINS. ONCE THESE PATHWAYS ARE MORE CLEARLY UNDERSTOOD, THE USE OF BIOCHAR APPLICATION TO SOIL AS A STRATEGY FOR REDUCING THE SPREAD OF ANTIBIOTIC COMPOUNDS AND ANTIBIOTIC RESISTANCE WITHIN THE AGRICULTURAL SYSTEM WILL BE ASSESSED.THE RESEARCH RESULTS WILL BE THEN BE USED IN NUMERICAL MODELS TOQUANTIFYTHE POTENTIAL RISKS OF ANTIMICROBIAL RESISTANCE WITHIN ANIMAL AND HUMANFOOD CHAINS. THIS WORK WILL HELP PROTECT HUMAN HEALTH FROM THE POTENTIAL RISKS OF ANTIBIOTIC RESISTANCE.