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**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** CRITICAL ADAPTATION GENES ARE OFTEN RAPIDLY EVOLVING AND CHANGING, INCLUDING GENES THAT CONTROL A CROPS NUTRITIONAL VALUE. THESE GENES OFTEN DISPLAYPRESENCE/ABSENCE VARIATION WHEREBY THE GENE IS PRESENT IN ONE PLANT BUT NOT ANOTHER. AT ONE LEVEL, THIS COMPLICATES TYPICAL GENOMIC APPROACHES TO FIND GENES THAT OFTEN ASSUME THAT A GENE IS PRESENT IN ALL GENOTYPES. THIS HOWEVER OPENS UP THE ABILITY TO COMPARE A GENES PRESENCE/ABSENCE WITH A NUTRIENTS PRESENCE/ABSENCE ACROSS PLANTS TO FIND THE GENES RESPONSIBLE FOR THE NUTRIENTS. TO TEST THE POTENTIAL FOR THIS TO AID IN CROP IMPROVEMENT, THIS PROPOSAL WILL WORK TO USE PRESENCE/ABSNCE VARIATION TO FIND THE ANTI-NUTRITIVE GS-OH WITHIN A MULTITUDE OF CRUCIFEROUS CROPS. GS-OH CREATES THE PROGOITRIN GLUCOSINOLATE THAT CAN LEAD TO THE ACCUMULATION OF GOITRIN, ISA KEY ANTI-NUTRIENT IN A WIDE RANGE OF CRUCIFEROUS CROPS INCLUDINGCRAMBE, RAPESEED, RADISH AND NUMEROUS BRASSICA FAMILY VEGETABLES LIKE BRUSSELS SPROUTS AND CABBAGES. REMOVAL OF THIS COMPOUND WAS A KEY REASON FOR THE CREATION OF THE TRIPLE ZERO CANOLA TO REPLACE RAPESEED. WHILE GS-OH WAS CLONED MODEL PLANTS, IT HAS BEEN RECALCITRANT TO IDENTIFICATION IN THESE OTHER CROPS. BY COMPARING THE PRESENCE/ABSENCE OF GENES IN THESE CROPS TO THE PRESENCE/ABSENCE OF PROGOITRIN, WE WILL CLONE AND VALIDATE THE GS-OH GENE IN EACH LINEAGE. THIS WILL ALLOW US TO REMOVE THIS GENE FROM THESE CROPS TO IMPROVE THEIR NUTRITIONAL VALUE. THIS PROJECT WILL ALSODEVELOP AND TEST APPROACHES TO FACILITATE IDENTIFYING KEY NUTRITIONAL AND BIOTIC GENES.

$500,000FY2020National Institute of Food and AgricultureUSDA

University Of California, Davis

Investigators

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**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** CRITICAL ADAPTATION GENES ARE OFTEN RAPIDLY EVOLVING AND CHANGING, INCLUDING GENES THAT CONTROL A CROPS NUTRITIONAL VALUE. THESE GENES OFTEN DISPLAYPRESENCE/ABSENCE VARIATION WHEREBY THE GENE IS PRESENT IN ONE PLANT BUT NOT ANOTHER. AT ONE LEVEL, THIS COMPLICATES TYPICAL GENOMIC APPROACHES TO FIND GENES THAT OFTEN ASSUME THAT A GENE IS PRESENT IN ALL GENOTYPES. THIS HOWEVER OPENS UP THE ABILITY TO COMPARE A GENES PRESENCE/ABSENCE WITH A NUTRIENTS PRESENCE/ABSENCE ACROSS PLANTS TO FIND THE GENES RESPONSIBLE FOR THE NUTRIENTS. TO TEST THE POTENTIAL FOR THIS TO AID IN CROP IMPROVEMENT, THIS PROPOSAL WILL WORK TO USE PRESENCE/ABSNCE VARIATION TO FIND THE ANTI-NUTRITIVE GS-OH WITHIN A MULTITUDE OF CRUCIFEROUS CROPS. GS-OH CREATES THE PROGOITRIN GLUCOSINOLATE THAT CAN LEAD TO THE ACCUMULATION OF GOITRIN, ISA KEY ANTI-NUTRIENT IN A WIDE RANGE OF CRUCIFEROUS CROPS INCLUDINGCRAMBE, RAPESEED, RADISH AND NUMEROUS BRASSICA FAMILY VEGETABLES LIKE BRUSSELS SPROUTS AND CABBAGES. REMOVAL OF THIS COMPOUND WAS A KEY REASON FOR THE CREATION OF THE TRIPLE ZERO CANOLA TO REPLACE RAPESEED. WHILE GS-OH WAS CLONED MODEL PLANTS, IT HAS BEEN RECALCITRANT TO IDENTIFICATION IN THESE OTHER CROPS. BY COMPARING THE PRESENCE/ABSENCE OF GENES IN THESE CROPS TO THE PRESENCE/ABSENCE OF PROGOITRIN, WE WILL CLONE AND VALIDATE THE GS-OH GENE IN EACH LINEAGE. THIS WILL ALLOW US TO REMOVE THIS GENE FROM THESE CROPS TO IMPROVE THEIR NUTRITIONAL VALUE. THIS PROJECT WILL ALSODEVELOP AND TEST APPROACHES TO FACILITATE IDENTIFYING KEY NUTRITIONAL AND BIOTIC GENES. · GrantIndex