THIS PROJECT SEEKS TO IMPROVE THE EFFICIENCY OF PRODUCING NON-MOSAIC KNOCKOUTS, AND KNOCK-INS, USING GENOME EDITING IN FOUR MAMMALIAN FOOD ANIMAL SPECIES (BOVINE, OVINE, CAPRINE AND PORCINE). THE PROPOSED APPROACH SEEKS TO AVOID THE INEFFICIENCIES ASSOCIATED WITH THE USE OF SCNT, CPI OF EDITING REAGENTS INTO ZYGOTES, AND THE PRODUCTION OF MOSAIC OFFSPRING. THESE METHODOLOGIES AND THEIR INHERENT PROBLEMS CURRENTLY LIMIT THE SCALABILITY OF GENOME EDITING IN LIVESTOCK. OUTCOMES OF THIS PROPOSAL WILL INCLUDE AN ELECTROPORATION-BASED PROTOCOL FOR THE RAPID INTRODUCTION OF INTENDED GENOMIC ALTERATIONS VIA GENOME EDITING IN FOUR MAJOR MAMMALIAN FOOD ANIMAL (BOVINE, OVINE, CAPRINE AND PORCINE) SPECIES. OUR ABILITY TO WORK IN FOUR SPECIES WILL SPEED APPLICATION OF RESULTS IN THE MAIN LIVESTOCK SPECIES.

University Of California, Davis

Investigators