THE OVERALL GOAL OF THIS PROJECT IS TO ESTABLISH TWO COMPLEMENTARY HIGH INTENSITY PHENOTYPING SITES AT UNIVERSITY OF NEBRASKA-LINCOLN (UNL) AND TEXAS A&AMP;M UNIVERSITY AT COLLEGE STATION, TX (TAMU), FOCUSING ON MAIZE AND WHEAT. OUR FOUR SPECIFIC OBJECTIVES ARE: (1) SUPPORT TWO ONGOING COMMUNITY-BASED PLANT PHENOTYPING EFFORTS AT BOTH UNL AND TAMU; NAMELY, THE MAIZE GENOMES TO FIELDS INITIATIVE (G2F) AND THE WINTER WHEAT BREEDING PROGRAMS; (2) ADVANCE AND APPLY UNMANNED AERIAL VEHICLES (UAVS) AS OUR MAIN PLATFORM FOR SENSOR DEPLOYMENT AND DATA COLLECTION, FOCUSING ON THE DEVELOPMENT OF NEW SENSORS AND TOOLS, AS WELL AS PRECISE AND CONSISTENT SYSTEMS AND PROTOCOLS, TO ENABLE HIGH THROUGHPUT ANALYSIS OF PLANT PHYSIOLOGICAL AND BIOCHEMICAL TRAITS AT LEAF AND CANOPY LEVELS; (3) DISSEMINATE PHENOTYPING DATA BROADLY AND PROMPTLY VIA PUBLIC DATA REPOSITORIES, AND CREATE IMAGE PROCESSING AND ANALYTICAL TOOLS THAT PROVIDE END-TO-END SOLUTIONS TO EXTRACT TARGET TRAITS FROM UAV IMAGES; (4) TRAIN A NEXT GENERATION WORKFORCE IN HIGH THROUGHPUT PLANT PHENOTYPING THROUGH GRADUATE AND UNDERGRADUATE RESEARCH AND BY DEVELOPING IN-CLASS CURRICULUM, ONLINE EDUCATIONAL MATERIAL, AND SUMMER SHORT-TERM TRAINING WORKSHOPS. OVER THE LIFE OF THE PROJECT, WE ANTICIPATE MAKING LEAPS IN THE SCIENCE AND ENGINEERING OF PLANT PHENOTYPING, INCLUDING NEW SENSOR/TOOL DEVELOPMENT, DATA MODELING AND ANALYTICS, AND WORKFORCE DEVELOPMENT. IN THE LONG TERM, WE SEEK TO REPLICATE OUR GAINS BY ESTABLISHING A NETWORK OF HIGH THROUGHPUT PHENOTYPING SITES NATIONWIDE WITH WHAT WE AND OTHERS HAVE LEARNED.