NATURALLY-SUPPRESSIVE SOILS PROVIDE STRONG EVIDENCE OF THE POTENTIAL FOR INDIGENOUS SOIL MICROBES TO DELIVER SIGNIFICANT, STABLE, AND LONG-LASTING DISEASE CONTROL IN AGRICULTURAL PRODUCTION SYSTEMS. HOWEVER, PRACTICAL MANAGEMENT TO ACHIEVE CONSISTENT DISEASE SUPPRESSION HAS BEEN ELUSIVE IN AGRICULTURAL SYSTEMS. HERE WE PROPOSE TO UTILIZE A NOVEL COEVOLUTIONARY FRAMEWORK FOR STUDYING THE GENERALIZABILITY OF ECOLOGICAL AND EVOLUTIONARY MECHANISMS FOR GENERATING PATHOGEN-SUPPRESSIVE MICROBIOMES AMONG DISTINCT CROP MANAGEMENT SYSTEMS. IN ADDITION, WE WILL EXPLORE THE SPECIFIC ROLES THAT ANTIBIOTICS PLAY IN MEDIATING PATHOGEN-ANTAGONIST INTERACTIONS AND DISEASE SUPPRESSION, AND CHARACTERIZE THE NETWORKS OF ANTIBIOTIC-MEDIATED SPECIES INTERACTIONS ACROSS SUPPRESSIVE SOIL MICROBIOMES. FINALLY, WE WILL CHARACTERIZE CO-ASSOCIATION AND INTERACTION NETWORKS FOR BACTERIAL AND FUNGAL MICROBIOMES FROM SUPPRESSIVE SOILS INDUCED BY DISTINCT CROP MANAGEMENT SYSTEMS TO IDENTIFY CONSISTENT FINGERPRINTS' OF SUPPRESSIVE SOIL MICROBIOMES. THIS PROJECT WILL ADDRESS KEY PROGRAM AREA PRIORITIES OF PROMOTING HEALTHY POPULATIONS OF BENEFICIAL ORGANISMS, AND INCREASING OUR UNDERSTANDING OF INTERACTIONS BETWEEN PESTS AND BENEFICIAL MICRO-ORGANISMS. IN ADDITION, THIS FUNDAMENTAL RESEARCH WILL CONTRIBUTE TO THE DEVELOPMENT OF BIOLOGICALLY-BASED PEST MANAGEMENT APPROACHES TO SUSTAIN BENEFICIAL SPECIES AND MANAGE PESTS, AND PROVIDE NOVEL INSIGHTS INTO THE BASES FOR PATHOGEN SUPPRESSION BY INDIGENOUS SOIL MICROBES. IN TOTAL, THE PROPOSED WORK WILL CONTRIBUTE SIGNIFICANTLY TO ADVANCES IN OUR POTENTIAL TO MANAGE SOIL MICROBIOMES TO ACHIEVE DISEASE SUPPRESSION, CONTRIBUTING TO INCREASES IN PLANT PRODUCTIVITY, REDUCTIONS IN RELIANCE ON PESTICIDES, AND MORE SUSTAINABLE FOOD PRODUCTION.