ANTIMICROBIAL RESISTANCE (AMR) RESEARCH, OUTREACH, AND COMMUNICATION INVOLVE MULTIPLE STAKEHOLDERS, YET, TO DATE, THERE HAS NOT BEEN A SYSTEMATIC CHARACTERIZATION OF AMR PERCEPTIONS AND KNOWLEDGE, OR EFFECTIVE DEVELOPMENT OF AMR COMMUNICATION STRATEGIES. IN ADDITION, THERE IS EVIDENCE SUGGESTING THAT THERMAL-BASED PROCESSES ARE MORE EFFECTIVE IN REDUCING AMR, HOWEVER, THE RELATIONSHIP BETWEEN FARM-APPROPRIATE, HIGHER-TEMPERATURE MANURE TREATMENT METHODS AND AMR REDUCTIONS HAVE NOT BEEN SPECIFICALLY QUANTIFIED. THIS RESEARCH WILL ADDRESS THESE NEEDS BY EVALUATING AMR PERCEPTIONS AND CREATING TARGETED OUTREACH CAMPAIGNS FOR FARMERS, POLICYMAKERS AND OTHER KEY STAKEHOLDERS. WE WILL ALSO DETERMINE MANURE MATRIX VARIABILITY AND CREATE A ROBUST. SCIENCE-BASED, MANURE SAMPLING PROTOCOL FOR AMR TO ADDRESS THE INHERENT VARIABILITY OF THE MANURE MATRIXES AND TEST HIGHER-TEMPERATURE MANURE TREATMENT METHODS USING THE PROJECT-DEVELOPED PROTOCOL. THE LONG-TERM GOALS OF THIS PROJECT ARE TO: 1) PROVIDE RESEARCH-BASED KNOWLEDGE OF STAKEHOLDER PERCEPTIONS (AND MISCONCEPTIONS) OF AMR PREVALENCE, CONVEYANCE, AND REDUCTIONS IN THE ENVIRONMENT, 2) CREATE AND IMPLEMENT COMMUNICATION STRATEGIES AND TOOLS THAT BEST CONVEY AMR KNOWLEDGE, AND 3) RESEARCH THE MOST PROMISING THERMAL-BASED AMR MANURE TREATMENT TECHNOLOGIES IN DAIRY OPERATIONS BASED ON OUR CREATED DAIRY MANURE SAMPLING PROTOCOL FOR AMR.THE RESULTS FROM THIS PROJECT CAN BE USED TO INFORM FARMERS ON MANURE MANAGEMENT TECHNOLOGIES AND HOW TO EFFECTIVELY CONVEY AMR KNOWLEDGE. THE TEAM HAS UNIQUE EXPERIENCE WITH MEDIA OUTREACH AND WILL DISSEMINATE SCIENTIFIC RESEARCH EFFECTIVELY THROUGH ITERATIVE DESIGN INVOLVING STAKEHOLDER REVIEW AND FEEDBACK, DISCUSSIONS USING ONLINE SOCIAL MEDIA, REINFORCING MESSAGES WITH IN-DEPTH WEB-BASED INFORMATION, AND IN-PERSON PRESENTATIONS AND DISCUSSIONS WITH KEY STAKEHOLDERS.