THIS GROUND-BASED PROPOSAL WILL EVALUATE A NOVEL SELF-GENERATED LOWER BODY NEGATIVE PRESSURE (LBNP) DEVICE AS A COUNTERMEASURE TO PREVENT SPACEFLIGHT-ASSOCIATED NEURO-OCULAR SYNDROME (SANS). THIS DEVICE IS IDEAL FOR OFF-NORMAL CONDITIONS IN SPACE CRAFT WHEN POWER IS LOW. THE SELF-GENERATED LBNP DEVICE WAS INVENTED AND PUBLISHED BY OUR TEAM ALMOST 20 YEARS AGO AND IS PRESENTLY ON THE CHINESE SPACE STATION TIANGONG ONE. THIS CONCEPT IS VERY TIMELY NOW WITH NASAS NEED TO PROVIDE A SANS COUNTERMEASURE WHILE AT THE SAME TIME PROVIDING PHYSIOLOGICALLY-INTEGRATED EXERCISE HARDWARE THAT IS SAFE LOW MASS LOW VOLUME LOW POWER AND SIMPLE FOR DEPLOYMENT ON A SMALL CONFINED DEEP-SPACE VEHICLE. PREVIOUS GROUND-BASED TESTS OF THE SELF-GENERATED LBNP DEVICE DOCUMENT THAT THE MAXIMUM FOOTWARD FORCE AT THE PEAK OF TBE EXERCISE CYCLE IS OVER 110 KG AND PRESSURE WITHIN THE CYLINDER CONCOMITANTLY DECREASES BY OVER 25 MM HG BELOW AMBIENT TO HELP COUNTERACT SANS MAINTAIN AEROBIC CAPACITY AND THE MUSCULOSLELETAL SYSTEM. THIS PROPOSAL IS A LOGICAL EXTENSION OF OUR PREVIOUS GROUND-BASED SIMULATIONS VALIDATING THE SELF-GENERATED LBNP DEVICE TO RE-INTRODUCE DAILY GRAVITATIONAL PRESSURES AND FOOTWARD REACTION FORCES. FURTHERMORE IT EXTENDS OUR ONGOING ISS PROJECTFLUID DISTRIBUTION BEFORE DURING AND AFTER PROLONGED SPACE FLIGHT DEMONSTRATING SHORT-TERM LBNP BY THE RUSSIAN CHIBIS SUIT TO REDUCE VENOUS CONGESTION IN THE NECK. WE WILL USE STATE-OF-THE-ART NON-INVASIVE TECHNOLOGIES AND IMAGING TO PROVE EFFICACY OF OUR SELF-GENERATED LBNP DEVICE BY QUANTIFYING CEREBRAL VOLUMES PRESSURES AND COMPLIANCE ALONG WITH VISUAL DEFICITS AND OCULAR REMODELING IN 16 HEALTHY FEMALE AND MALE VOLUNTEERS DURING PARABOLIC FLIGHT AND GROUND SIMULATIONS OF MICROGRAVITY. WE WILL DETERMINE DOSE-RESPONSE EFFICACY OF SELF-GENERATED LBNP AND ACCOMPANYING SHOULDER-VEST AND FOOTWARD MECHANICAL LOADS TO RE-INTRODUCE DIURNAL EFFECTS OF GRAVITATIONAL STRESS. OUR SELF-GENERATED LBNP DEVICE IS VERY TIMELY NOW WITH NASAS NEED TO PROVIDE AN INTEGRATED COUNTERMEASURE FOR SANS AND MS LOSSES WHILE AT THE SAME TIME PROVIDING PHYSIOLOGICALLY-INTEGRATED EXERCISE HARDWARE THAT IS SAFE LOW MASS LOW VOLUME NO POWER AND SIMPLE FOR DEPLOYMENT IN A CONFINED DEEP-SPACE VEHICLE. TAKEN TOGETHER WE THEREFORE PROPOSE LOW-LEVEL ALMOST DAILY APPLICATION OF SELF-GENERATED LBNP AS AN INTEGRATED COUNTERMEASURE TO REINTRODUCE DIURNAL CYCLES OF GRAVITATIONAL FLUID AND PRESSURE VARIABILITY TO PRESERVE CEREBRAL OCULAR CARDIOVASCULAR AND MUSCULOSKELETAL HEALTH RELEVANT TO 2011 DECADAL PRIORITIES AH6 AND CC2. A SELF-GENERATED LBNP DEVICE WILL GO FROM A TECHNOLOGY READINESS LEVEL OF 7 OR 8 TO 9. EXERCISE WITHIN LBNP IS AT COUNTERMEASURE READINESS LEVEL OF 7 TO 8 OR 9.