OUR RESULTS WILL GO BEYOND MICROBIAL MEASUREMENT TO PREDICT MOISTURE-RELATED CHANGES TO THE INDOOR MICROBIOME AND ASTRONAUT EXPOSURE. OUR WORK WILL PROVIDE ADDED VALUE TO MICROBIAL DATA COLLECTED PREVIOUSLY ON THE ISS. FOR INSTANCE MICROBIAL TRACKING 1 CHARACTERIZED AIRBORNE AND SURFACE-ASSOCIATED POPULATIONS OF MICROORGANISMS ABOARD THE ISS. HUMID COMPLEMENTS THIS DATA THROUGH USE OF A CHAMBER STUDY OBSERVING FUNDAMENTAL MICROBIAL PROCESSES THAT MAY OCCUR UNDER CONDITIONS OF INCREASED MOISTURE IN OCCUPIED SPACECRAFT. WE NEED TO UNDERSTAND HOW TO CHARACTERIZE THE SPACECRAFT MICROBIOME AS EITHER HEALTHY OR UNHEALTHY. ULTIMATELY WE WILL BE ABLE TO DEVELOP IMPROVED MICROBIAL CLEARANCE PROTOCOLS THAT ARE MORE RELEVANT TO ASTRONAUT HEALTH PLANETARY PROTECTION AND SPACECRAFT INTEGRITY. OUR DESIGN DECISIONS IN SPACE HAVE IMPORTANT IMPLICATIONS IN CONTROLLING THE MICROBES THAT ARE PRESENT. BY UNDERSTANDING THE FUNDAMENTAL PROCESSES THAT OCCUR IN THIS ENVIRONMENT WE CAN DESIGN FOR A HEALTHY MICROBIOME INSTEAD OF BEING SUBJECT TO UNINTENDED CONSEQUENCES.