IN-ORBIT OR ON-SURFACE CRYOGENIC STORAGE FOR PROPELLANT AND LIFE SUPPORT CONSUMABLES IS ESSENTIAL TO ALL STAGES OF NASA S EXPLORATION PROGRAM. HOWEVER HEAT LEAKAGE FROM THE SURROUNDINGS RESULTS IN LIQUID VAPORIZATION AND SIGNIFICANT MASS LOSS AND/OR SIGNIFICANT INCREASE IN THE STORAGE TANK PRESSURE. TO MITIGATE THE UNWANTED EFFECTS OF CRYOGEN VAPORIZATION THE ZERO BOIL-OFF (ZBO) CONCEPT IS BEING CONSIDERED AS AN INNOVATIVE APPROACH FOR VENTLESS MEANS OF CONTROLLING TANK PRESSURE THROUGH THE APPLICATION OF ACTIVE HEAT REMOVAL AND FORCED LIQUID JET MIXING. THE ZERO BOIL-OFF TANK (ZBOT) EXPERIMENT IS DESIGNED FOR OPERATION ON THE INTERNATIONAL SPACE STATION (ISS). ITS PURPOSE IS TO EXAMINE THE ZBO CONCEPT AND ALONG WITH COMPUTATIONAL MODELING EFFORTS PROVIDE INFORMATION ON THE FUNDAMENTAL PHYSICS OF THE UNDERLYING COMPLEX THERMOFLUIDS ENVIRONMENT WHICH IS NEEDED FOR ENGINEERING DESIGN OPTIMIZATION OF THE ZBO CONCEPT. IN AN EFFORT THAT WAS LAUNCHED NEARLY TWO YEARS AGO UNDER NASA SUPPORT (GRANT NO. NNX16AD52A) WE HAVE SUCCESSFULLY DEMONSTRATED THE FEASIBILITY OF NON-INTRUSIVE MOLECULARBASED TEMPERATURE AND VELOCITY MEASUREMENT METHODS SUITABLE FOR THE ZBOT MICROGRAVITY EXPERIMENT. MOLECULAR TAGGING VELOCIMETRY (MTV) ADDS COMPLEMENTARY CAPABILITY TO THE DIGITAL PARTICLE IMAGE VELOCIMETRY (DPIV) CURRENTLY USED IN THE ZBOT EXPERIMENT WHEREAS LASER INDUCED FLUORESCENCE (LIF) THERMOMETRY BASED ON SOLUBILIZED QUANTUM DOTS (QD) BRINGS IN WHOLE-FIELD THERMOMETRY CAPABILITY ONE THAT DID NOT EXIST PREVIOUSLY. THE OBJECTIVES OF THE PROPOSED RESEARCH ARE TO CAPITALIZE ON OUR PROGRESS TO DATE IN THERMOMETRY DIAGNOSTICS AND MORE FULLY QUANTIFY THE VARIOUS INTERCONNECTED PARAMETERS AND THE TRADEOFFS AMONG THEM THAT INFLUENCE THE ABILITY TO MAKE ACCURATE TEMPERATURE MEASUREMENTS. THIS TYPE OF INFORMATION IS NEEDED TO OPTIMIZE THE DESIGN OF EXPERIMENTS FOR ACCURATE QUANTITATIVE MEASUREMENTS IN THE ACTUAL ZBOT TEST SECTION ON THE ISS.