SCIENCE:.. UTILIZE BUBBLE-BASED SCRUBBING AND BURSTING TO.CONCENTRATION ORGANIC MATERIAL UP TO 1000X IN LIQUID.DERIVED FROM ICE BRINE OR SUB-SURFACE OCEAN.. ENABLE LIFE DETECTION AT 1-10 CELLS/ML ABUNDANCE.. IMPROVE LIMIT OF DETECTION (LOD) OF LIFE DETECTION.INSTRUMENTS TARGETING AMINO ACIDS (CHIRAL EXCESS .COMPLEXITY-ABUNDANCE DISTRIBUTION NUCLEIC.ACIDS/INFORMATIONAL POLYMERS WHOLE CELLS.(MICROSCOPY MOBILITY).OBJECTIVES:.. CONDUCT LAB EXPERIMENTS TO DETERMINE OPTIMAL.SYSTEM DESIGN.. DEVELOP PROTOTYPE INSTRUMENT AND EVALUATE.PERFORMANCE ON A SET OF OCEAN WORLD ANALOGS.. ADVANCE TECHNOLOGY READINESS LEVEL (TRL) FROM.TRL 2 TO TRL 4 AND CHARACTERIZE STEPS REQUIRED TO.ADVANCE BOOST WITH FURTHER FUNDING TO TRL 6.KEY MILESTONES:.. EXPERIMENTAL CONTROL AND OPTIMIZATION OF ENRICHMENT.DEMONSTRATED (YEAR 1) AND PROTOTYPE DEVELOPED (YEAR 2).. ENRICHMENT EVALUATED IN IDEALIZED OCEAN WORLD SAMPLES.FROM MOLECULES TO CELLS THRESHOLD 102 TARGET 103 (YEAR 2).. FULL SCALE EVALUATION OF LIFE DETECTION LOD IMPROVEMENTS.USING ENVIRONMENTAL ANALOGS OF OCEAN WORLDS (YEAR 3).