INVIZYNE TECHNOLOGIES, INC., NEW SBIR AWARD UNDER FOA NUMBER DE-FOA-0001954 (SEED) WITH PROJECT TITLE ''SIMPLIFYING REACTOR SETUP FOR CELL-FREE BIOFUEL PRODUCTION'' TO USE DEMONSTRATED PRODUCTION OF THE 2ND GENERATION BIOFUEL ISOBUTANOL TO IMPROVE THESE PRODUCTION PARAMETERS SIGNIFICANTLY AND HAVE VIRTUALLY EXTINGUISHED A MAJOR CONTRIBUTOR TO COGS BY DEVELOPING METHODS TO OBTAIN 10X CHEAPER COFACTORS. INVIZYNE PROPOSE TO DEVELOP A NEW METHOD, OF CELL GROW/INDUCE TAGGED PATHWAYS, TO SIMPLIFY AND REDUCE THE COST OF ENZYME ISOLATION.