CAREER: Dissecting Snow Algal Community Interactions to Understand Environmental Change Impacts

Carroll College, Helena MT

Investigators

Abstract

Algae blooming on snow, ice, and glaciers (commonly called pink snow) accelerate melting due to the absorbance of light by their dark pigments. With rising temperatures, additional contributions to glacier and snow melt are becoming of greater concern. Snow algae grow in communities with other microorganisms such as bacteria and fungi. Currently, little is known about the interactions between algal and non-algal species or how they respond to environmental conditions. This project investigates snow algal community in Montana using a bottom-up approach, first characterizing the makeup of snow algal communities and isolating members for controlled laboratory growth experiments, leading to development of a model detailing how individual species contribute to community growth. By sampling snow algal communities in the same locations over five years, this project will gather important information about the seasonal and annual dynamics of snow algae. This CAREER project will involve National Forest and Park partnerships, integrate undergraduate researchers in the research process both inside and outside of the classroom, and engage rural Montana middle schools with the data collection process and underlying scientific concepts. The results of this project are expected to advance current understanding of snow algal community composition, function, and response to changing environments. Gaining an understanding of how snow algal communities operate is anticipated to also aid in devising strategies to better manage their effects on snow melt. The overarching goal is to develop an experimentally grounded model system of snow algal communities predicting their responses to key environmental variables, such as light and nutrient availability. To develop a metabolic scale understanding of interspecies interactions, three major approaches will be used to investigate snow algal communities in Glacier National Park and the Beartooth Mountains in Montana: (1) characterize snow algal community structure and identify genetic potential through Nanopore DNA sequence profiling, (2) develop a representative snow algal community metabolic model from genomic information and predict responses to key environmental variables through computational analysis of metabolic pathways, and (3) cultivate select algal species under laboratory conditions to test responses to environmental factors and provide feedback to refine the model. Each of these objectives is intertwined with undergraduate education through bringing research into course-based labs and offering individualized summer research experiences. This work will advance current understanding of microbial interactions in snow algal communities and gain insight into ecosystem impacts; understanding productivity and stability over time in response to changing environmental factors is essential to predict how snow algae may affect the global climate crisis in years ahead. The research process will establish a structure for transforming large environmental genomic data sets into predictive models, helping to both describe and quantify microbial interactions and nutrient cycling in response to environmental stressors. This project is jointly funded by the Population and Community Ecology Cluster, Division of Environmental Biology and the Established Program to Stimulate Competitive Research (EPSCoR) and the Systems and Synthetic Biology Cluster in the Division of Molecular and Cellular Biosciences. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.