CAREER:Three-dimensional, super-resolution, and super-sensitivity quantitative molecular multiphoton microscopy in living tissue

University Of Notre Dame, Notre Dame IN

Investigators

Abstract

ABSTRACT PI: Howard, Scott Proposal: 1554027 The goal of this CAREER proposal is to study, develop, characterize, and evaluate a fundamentally new method for super-resolution molecular imaging in living animals. An approach capable of visualizing functional and morphological markers of pathological conditions located deep within the tissue would be a breakthrough in optical imaging and its applications in biomedical research and the development of novel therapeutic and diagnostic approaches. Significant efforts will be dedicated to teaching translational applications of biophotonics with emphasis on helping students develop entrepreneurship skills in the field of biophonics and imaging. This proposal will provide researchers, for the first time, a platform for three-dimensional, super-resolution imaging deep in living animals while simultaneously providing super-sensitivity molecular measurements in the cellular microenvironment. This new super resolution molecular imaging modality is based on analyzing harmonic distortions in a multiphoton microscope. This is an ambitious goal and the idea of using saturation to further localize fluorescence signals is innovative.