CAREER: Engineering and Integrated Microfluidic System for High Throughput Genomic Analyses

University Of California-San Diego, La Jolla CA

Investigators

Abstract

0547193 Huang The overall goal of this research is to develop a multidisciplinary program to create such a new paradigm with two specific objectives: (1) To engineer microfluidic devices for whole genome-scale DNA amplification, and (2) To develop an integrated system for high throughput genome sequencing and digital gene expression profiling. Microfabrication tools will be used to engineer high density arrays of femtoliter wells on polydimethylsiloxane (PDMS) substrates for whole genome-scale DNA amplification. Single DNA molecules from a genomic DNA or cDNA library will be cloned in massive parallel on the microfluidic devices using the rolling circle amplification (RCA) technology developed by the investigator. A new sequencing technology called "sequencing by denaturation" (SBD) will be developed to enable the massive parallel sequencing of the single molecular clones on the whole genome or transcriptome array. These technologies will be integrated with a high throughput imaging system to engineer an integrated system for genomic analyses.