Acquisition of an Agilent 1100 Series MALDI/LC TOF Mass Spectrometer for Three Collaborating Departments at Duquesne University

Duquesne University, Pittsburgh PA

Investigators

Abstract

With support from the Major Research Instrumentation (MRI) Program, the Department of Chemistry and Biochemistry at Duquesne University will acquire a Matrix-assisted Laser Desorption Ionization - Time of Flight (MALDI-TOF) Liquid-Chromatograph Mass Spectrometer. This instrument will be used in a wide variety of research projects, including studies on a) organic and inorganic synthesis reactions; b) correlating specific proteins with defined genetic activity; c) identifying and exploiting the bio-markers for discrete conditions or outcomes; and d) quantitative characterization of various types of surface modifications, from functionalized self assembled monolayers to fingerprints and genetic material. Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry is the technique of choice for obtaining accurate molecular weights on molecules up to and over 300,000 daltons, with extremely high sensitivity. Use of a MALDI-TOF mass spectrometer has therefore become a standard technique, especially in studies involving biomolecules. This research will have a significant impact in a number of areas, especially biochemistry, medicinal chemistry and materials science.