SGER: Cellular Interrogation Using Fiberoptic Nanobiosensors

Florida Institute Of Technology, Melbourne FL

Investigators

Abstract

0406282 Mitra The investigators will study the mechanism of laser therapy using fiberoptic nanobiosensors. Low intensity ultraviolet, visible and near and mid-infrared radiation will be carefully aimed and injected onto and through the cell membrane with tiny evanescent fiber and micropipette-like nanoprobes constructed for efficient delivery of the radiation onto sub-cellular organelles. Microprobes will be used to sense light by photon counting and the emission of chemicals will be sensed by mid-infrared absorption using evanescent tips. Responses could occur in the form of light emission such as luminescence from singlet oxygen or a chemical emission. A major goal is to determine the photochemical mechanism responsible for cell stimulation in the case of laser therapy and cell death in the case of photodynamic therapy.