CORE--MOLECULAR BIOLOGY

$130,719P50FY2002AANIH

Indiana Univ-Purdue Univ At Indianapolis, Indianapolis IN

Investigators

Linked publications & trials

Paper 39358829 Paper 39256902 Paper 38496601 Paper 38342379 Paper 37873153 Paper 37871677 Paper 37838352 Paper 37690674 Paper 37028106 Paper 36001429 Paper 34590334 Paper 33908131 Paper 33560411 Paper 33496972 Paper 32966634 Paper 32945559 Paper 32882352 Paper 32607619 Paper 32484507 Paper 32150428 Paper 31542609 Paper 31217828 Paper 31093721 Paper 31029878 Paper 30758525 Paper 30129253 Paper 30043172 Paper 29955167 Paper 29713788 Paper 29448234 Paper 29144544 Paper 29121399 Paper 28858384 Paper 28433420 Paper 28215999 Paper 28000083 Paper 27696522 Paper 27583369 Paper 27461754 Paper 27293152 Paper 27260326 Paper 27242276 Paper 27060486 Paper 27038599 Paper 26935871 Paper 26536631 Paper 26383016 Paper 26334550 Paper 26306917 Paper 26283074 Paper 26250465 Paper 26207767 Paper 26110891 Paper 26074425 Paper 25922425 Paper 25813708 Paper 25702586 Paper 25585681 Paper 25557834 Paper 25541501 Paper 25346508 Paper 25307596 Paper 25206361 Paper 25155311 Paper 25131541 Paper 25085719 Paper 25038869 Paper 25026094 Paper 24966868 Paper 24961433 Paper 24653733 Paper 24619539 Paper 24611993 Paper 24599396 Paper 24588897 Paper 24355552 Paper 24268381 Paper 24252444 Paper 24231358 Paper 24167711 Paper 24157127 Paper 24095931 Paper 23986666 Paper 23875623 Paper 23832875 Paper 23731093 Paper 23714385 Paper 23712313 Paper 23575833 Paper 23544149 Paper 23520600 Paper 23506787 Paper 23496648 Paper 23414322 Paper 23240885 Paper 23172699 Paper 23148856 Paper 23103403 Paper 22976430 Paper 22971542

Abstract

The overall goal of the Molecular Biology Core of this Alcohol Research Center is to support studies that pursue the genes underlying alcohol- seeking behavior, alcoholism, and related diseases. The Core has developed polymerase chain reaction (PCR) - based genotyping assays for the alcohol metabolizing enzymes ADH2, ADH3, ALDH2, and CYP2El and has determined the allele frequencies of these loci in various ethnic populations. We will continue to offer these genotyping services to investigators in order to determine if there are further relationships between these enzymes and responses to alcohol. The Core will also employ single strand length polymorphism and cycle sequencing assays to search for polymorphisms in the promoters of ADH1, ADH2, ADH3, ADH4, ADH7, ALDH1 and ALDH2. If a difference is found, we will develop a PCR-based genotyping assay. To complement our human studies, a new focus of the Molecular Biology Core will be the support of QTL mapping studies in animal lines selected for alcohol drinking. The rat studies will be expedited by performing marker heterozygosity screening in the N/Nih rats. Subsequent screening of the high (HARF) and low (LARF) drinking rats from the Addiction Research Foundation (ARF) are proposed for years 3 and later. The high (HAP1) and low (LAP1) alcohol preference mice, bred by the Mouse Selective Breeding Component, will be tested with markers in candidate regions identified in the recombinant inbred mice as possible locations for QTLs underlying alcohol preference.

View original record on NIH RePORTER →