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Collaborative Research: Production and Use of a Ligninolytic Enzyme for Environmentally Benign Paper Manufacturing (TSE03-K)

$142,000FY2003ENGNSF

Syracuse University, Syracuse NY

Investigators

Abstract

0328033 Kelly The objective of the proposed research is the development of methods for production and purification of the lignin-degrading enzyme, manganese peroxidase (rMnP), using the yeast Pichia pastoris in high cell density, fed-batch fermentations. The concentrated enzymes will be tested for their effectiveness in an environmentally benign manufacturing process for lignin removal during pulp and paper manufacturing.

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