CRYO COOLING OF XENON DERIVATIZED RNA POLYMERASE CRYSTALS FOR MIR PHASING

$143,176P41FY2002RRNIH

Stanford University, Stanford CA

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Abstract

Crystals of RNA polymerase were pressurized at high pressure xenon (50 atm) and then flash-cooled ~5 seconds after depressurization. The isomorphous difference Patterson map revealed a xenon site, although preliminary analysis indicates partial occupancy and low phasing power.

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