Development of On-line Flow Cytometry

University Of Minnesota-Twin Cities, Minneapolis MN

Investigators

Abstract

The objective of this project is to develop an on-line flow cytometry system. A sorting flow cytometer will be interfaced to a bioreactor. The interface will allow automatic sampling and staining of the cells. The primary application of the on-line flow cytometry system will be on monitoring culture physiology at the single-cell level. Specifically, experiments will be carried out to determine single cell glucose uptake rates for E. coli, the fraction of S. cerevisae cells undergoing mitosis and, finally, for the isolation of E. coli transport mutants.