Gene Expression and Microarrays

National Institute Of Environmental Health Sciences

Investigators

Linked publications, trials & patents

Abstract

The mission of the Molecular Genomics Core (MGC) is to utilize advances in genomic and bioinformatic technologies to enable Intramural NIEHS scientists to pursue studies that address how the environment influences human health. The MGC continuously strives to develop and implement new analytical and computational approaches to investigate genome-wide changes in gene expression. Specific technical accomplishments include testing and optimization of RNA isolation and amplification protocols to perform microarray experiments with RNA from limited tissue sources e.g. laser capture microdissected and embryonic samples. The MGC utilizes Affymetrix and Illumina platforms to explore genome-wide analyses such as DNA methylation, gene expression, and microRNA (miRNA) applications. Additionally, the group utilizes Nanostring technology to further enhance its capabilities. The installation and implementation of software packages for emerging and existing genomic technologies has greatly improved the bioinformatics capacity of the MGC. In addition, continuing modifications to the NIEHS Microarray Core custom in-house Microarray Project System (MAPS) have ensured that the MGC can sufficiently track and manage sample and experimental information related to microarray and Nanostring analyses. Finally, the MGC has continued to refine a rigorous QA/QC protocol incorporated into a standard deliverable that is distributed upon completion of a project.