Efficient Immortalization of Primary Human Keratinocytes

National Institute Of Allergy And Infectious Diseases

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Abstract

We have previously established a treatment that allows us to efficiently and conditionally immortalize primary human keratinocytes and other epithelial cells by culturing them in the presence of a ROCK inhibitor and with feeder fibroblasts. The technique is invaluable for the efficient procurement of cells from individuals with specific genetic and infectious diseases. In FY2025 we have cultured keratinocytes from individuals at the NIH clinical center to assist clinical investigations and have generated organoids and 3D tissue culture models to study skin biology and cancer.