Molecular Vector and Conditional ES Cell Clone Collection Validation and Maintenance Plan by theMMRRC at UC Davis

University Of California At Davis, Davis CA

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Abstract

Molecular Vector and Conditional ES Cell Clone Collection Validation and Maintenance Plan by the MMRRC at UC Davis ABSTRACT This application from the Mutant Mouse Resource and Research Center at the University of California, Davis (MMRRC) proposes the validation and maintenance for distribution of data, molecular vectors, and ES cell clones for 4,867 genes targeted as knockout-first alleles produced by the EUCOMM Program. These genes are unique to the set of conditionally-targeted genes produced by KOMP2 as part of the International Mouse Phenotyping Consortium (IMPC) and are not currently available or accessible by NIH-funded researchers. Through an administrative supplement awarded last year, 2,751 vector and ES cell lines of this collection were acquired and are being transferred to the MMRRC at UC Davis. Arrangements are underway for the transfer of the balance of 2,116 over the next several months. However, full validation will be required to make the collection available for dissemination to the research community. To accomplish this goal, the MMRRC will conduct a number of quality controls steps (e.g., data verification and alignment with biological resources, concentration and purity of molecular vectors, conditional allele-specific genetic screening, cell morphology and viability in culture, chimera production and germline transmission, fecundity and viability of live offspring) to fully verify the identity and validate the utility of the vector and cell resources, produce and publish Strain Detail Sheets, integrate the collection into the MMRRC inventory, and list the collection in the online MMRRC catalog. The cost to validate and maintain this resource is a very small fraction (~2%) of what it would cost to recreate it using CRISPR-mediated gene-targeting. Once fully validated and integrated into the existing MMRRC inventory, the collection will be available for distribution to the biomedical research community upon request, especially to NIH-funded investigators who continue to express interest in conditional, lacZ tagged alleles for their research. As a result of the activities funded by this administrative supplement, expansion of the MMRRC molecular vector and conditional allele resource will exert a sustained, powerful influence on research field(s) using conditional mouse models and the overall ORIP mission to support a variety of resource and research centers that develop animal models of human biology and disease for US biomedical researchers. The costs to execute this project are beyond the available support provided by the parent MMRRC grant to UC Davis. If awarded, funds will be used to complete this project during the current grant year (ending January 31, 2026).