VetLIRN: Expanding Capability and Capacity at the ADRDL

South Dakota State University, Brookings SD

Investigators

Abstract

PROJECT SUMMARY/ABSTRACT South Dakota State University’s (SDSU) Department of Veterinary and Biomedical Sciences (VBSD) and Animal Disease Research and Diagnostic Laboratory (ADRDL) is proposing to upgrade our 2 Illumina MiSeq instruments to Illumina MiSeq i100 (1) and i100 Plus (1) instruments. Illumina has announced the sunsetting of our current platform and in order to maintain Whole Genome Sequencing (WGS) capability and capacity for the Vet- LIRN network, we will need to update to new WGS technology. The i100 systems would also provide advantages in run time, disposable cost, and analyst safety. Specific aims are to acquire and validate the Illumina MiSeq i100 instrument for use in bacterial sequencing, principally those samples submitted to our laboratory for Vet-LIRN AMR WGS studies and investigations into petfood related illnesses. We also aim to acquire and validate the Illumina MiSeq i100 Plus instrument, which will allow for early detection of emerging and zoonotic pathogens. The instrument will be validated, with a focus on Highly Pathogenic Avian Influenza (HPAI) in milk, food, and feed samples.