CORE--PROTEIN PROTEIN INTERACTIONS

$0P30FY2002CANIH

Ctrc Research Foundation, San Antonio TX

Investigators

Linked publications & trials

Paper 39713861 Paper 39649174 Paper 39532876 Paper 39513699 Paper 39496512 Paper 39488787 Paper 39466842 Paper 39464140 Paper 39409924 Paper 39361026 Paper 39281865 Paper 39261729 Paper 39258975 Paper 39257997 Paper 39189979 Paper 39164784 Paper 39080341 Paper 39051998 Paper 39024100 Paper 39018351 Paper 38996030 Paper 38991178 Paper 38932463 Paper 38918608 Paper 38902147 Paper 38895251 Paper 38893160 Paper 38889005 Paper 38883782 Paper 38875929 Paper 38854050 Paper 38784962 Paper 38781073 Paper 38702648 Paper 38656940 Paper 38645769 Paper 38627469 Paper 38506232 Paper 38391571 Paper 38370127 Paper 38328068 Paper 38315992 Paper 38311721 Paper 38299827 Paper 38293097 Paper 38280869 Paper 38247878 Paper 38112466 Paper 38104552 Paper 38098745 Paper 38086903 Paper 38084915 Paper 38060011 Paper 38059785 Paper 38028432 Paper 38014186 Paper 37990009 Paper 37953292 Paper 37917239 Paper 37904250 Paper 37873441 Paper 37858985 Paper 37853941 Paper 37846664 Paper 37845223 Paper 37802546 Paper 37788996 Paper 37777742 Paper 37733728 Paper 37686559 Paper 37659079 Paper 37650863 Paper 37577610 Paper 37573456 Paper 37553340 Paper 37493258 Paper 37428793 Paper 37359725 Paper 37344589 Paper 37335291 Paper 37333188 Paper 37319247 Paper 37315790 Paper 37292941 Paper 37288969 Paper 37268395 Paper 37266578 Paper 37243594 Paper 37205429 Paper 37196159 Paper 37186298 Paper 37174684 Paper 37169919 Paper 37093199 Paper 37066199 Paper 37055441 Paper 36876140 Paper 36773293 Paper 36750097 Paper 36711730

Abstract

DESCRIPTION (provided by applicant): The Protein-Protein Interactions Core Facility combines the complimentary strengths of the Surface Plasmon Resonance Laboratory and the Analytical Ultracentrifugation Laboratory, each of which provides rigorous quantitative analysis of macromolecular interactions and dynamic behavior of complex systems. The Protein-Protein Interactions Core Facility is located within the UTHSCSA Department of Biochemistry. The Surface Plasmon Resonance Laboratory occupies a 400 square foot room, and the Analytical Ultracentrifugation Laboratory occupies an adjoining 400 square foot room. The Surface Plasmon Resonance Laboratory consists of two Biacore 3000 surface plasmon resonance instruments and ancillary computers. The instrumentation?s strengths include speed, sensitivity, small sample consumption and, most importantly, the binding events are measured as they occur in real time. Thus, precise information on the kinetics and affinity of the binding interactions can be determined without the need for purified proteins or detection labels. The Analytical Ultracentrifugation Laboratory consists of three Beckman XL-I analytical ultracentrifuges and ancillary computers. Each analytical ultracentrifuge is equipped with both interference and absorbance optical systems for detecting sedimentation of samples during real time. In addition, funds have recently been obtained to add a fluorescence optical detection system to one of the existing analytical ultracentrifuges. The analytical ultracentrifuge can examine sample purity, detect and characterize conformational changes, determine subunit stoichiometries, characterize assembly and disassembly mechanisms of supramolecular assemblies, and measure equilibrium constants and thermodynamic parameters for associating systems. This information is critically important for understanding macromolecular interactions involved in the molecular basis of cancer.

View original record on NIH RePORTER →