Large Animal Models Core

Univ Of North Carolina Chapel Hill, Chapel Hill NC

Investigators

Linked publications & trials

Abstract

PROJECT SUMMARY - LARGE ANIMAL MODELS CORE The Large Animal Models Core was originally developed in 2014 because of the recognition that small animal models have been inadequate to solving human health digestive disease problems. This is particularly true where key human anatomical features are not present in small animals, or where the size of the animal is important for model development. The core has developed models of Barrett’s esophagus and eosinophilic esophagitis (EoE) that can be monitored repeatedly under anesthesia via a human gastroscope. The Core has been involved in intestinal transplantation in the pig using intestinal storage devices developed for humans and surgical techniques that mirror human size and techniques. Studies of epithelial repair following ischemic injury may have implications for necrotizing enterocolitis. The Core creates easy access for investigators. The Core staff submits IACUCs, arranges for housing, schedules procedures, configures procedural space, and coordinates with other units such as imaging. Animals are followed by Core veterinarians and lab animal veterinarians. These tasks are routine at the College of Veterinary Medicine but would be impossible for investigators at Duke and UNC. The Core makes it possible for them to conduct large animal research. Although the Core currently serves a small number of members, it provides a remarkable scientific impact on their research which would not be possible without the Core. The specific aims of the core are: 1. To provide consultation with Core veterinarians to assess feasibility, logistics and study design thus facilitating the development and approval of IACUC protocols. 2. To assist in the procurement of animals from NC State farms at a substantial savings 3. To provide access to animal procedures involving anesthesia including both endoscopy and surgery with subsequent postoperative follow up. 4. To develop novel research technologies involving large animal models such as genetic manipulation. The Core directors (Blikslager and Gonzalez) have a demonstrated track record in digestive disease research and are board-certified veterinary specialists in surgery. Veterinary board-certified specialists in laboratory animal medicine, internal medicine, anesthesia, and advanced imaging (MRI, CT, nuclear medicine) from the NC State Veterinary Hospital have all provided consultation over the last 10 years. Their collaboration has led to adaptations in anesthetic technique to resemble human procedures more closely, particularly post- procedural management. Specialists provide pain management and close monitoring when animals are returned to their housing to ensure optimal care using the Guide for the Care and Use of Agricultural Animals in Research in AAALAC-accredited facilities.