VAST Center: Viromes Across Space(s) and Time

Stanford University, Stanford CA

Investigators

Abstract

BIOSPECIMEN COLLECTION CORE ABSTRACT The VAST Center Biospecimen Collection Core (BCC) will actively collect, process, and store samples across the human lifespan, using innovative cross-validated Standard Operating Procedures (SOPs) for seamless integration. Collaborating with the other cores, we will prioritize identifying and encrypting crucial metadata by employing secure and ELSI-informed practices for data access, sharing, and reusability. We are uniquely positioned to carry this out, with several pre-existing cohorts - including some that span up to 10 years - diverse landscapes, and age groups; furthermore, our collection encompasses various populations, enabling a comprehensive understanding of the virome. Led by internationally recognized experts, the BCC Core collaborates closely with the ELSI core and PEDP to transparently address ethical concerns. Many cohorts will contribute specimens immediately upon award, with potential for additional sample collection through prior IRB- approved re-consenting. Aim 1 focuses on curating pre-existing specimens and metadata across 8 cohorts. The Virome Characterization Center (VCC) aims to construct a central repository database for pre-existing samples, serving the Biospecimen Analysis Core and Data Analysis Core, facilitating sample sharing. The VCC, with immediate access to well-characterized samples from diverse cohorts, is uniquely positioned for biospecimen analysis, including iPOP, Astronaut, Antarctica Travelers, Native American, African American & African, Kids Camp, Newborn, and Airborne Exposure cohorts. Rigorous recontact and reconsent mechanisms have been developed and are in alignment with the most rigorous ELSI standards. Aim 2 actively develops SOPs for future biospecimen collection, prioritizing plasma and stool. Sub-aims include defining biospecimen type, volume, source, frequency, and sampling approach; standardizing and harmonizing collection within and across cohorts; ensuring rigor in biospecimen and data collection and storage; developing optimal sample collection strategies for understudied body sites; and completing sample collection based on optimal procedures and size estimation. Aim 3 emphasizes maximizing informed consent breadth. With a proven track record, exemplified in the iPOP and Native American studies, we collaborate closely with communities to design ethical consent forms, ensuring timely sample and data sharing, aligning with the Human Virome Program's objectives. Our collaboration with the ELSI core and institutional review boards underscores our commitment to participant privacy, autonomy, and ethical virome characterization. In summary, our active approach, cross-validated SOPs, and ethical practices uniquely position our core to significantly contribute to the goals of the HVP.