Proteomics Core

$250,220P20FY2025GMNIH

University Of Oklahoma Hlth Sciences Ctr, Oklahoma City OK

Investigators

Linked publications, trials & patents

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Abstract

OK-INBRE PROTEOMICS CORE SUMMARY The goal of the OK-INBRE Proteomics Core is to provide researchers in Oklahoma access to state-of-the-art instrumentation and expertise for advanced mass spectrometry. The core is organized with the instrumentation and expertise required to make discovery proteomics and targeted quantitative proteomics experiments available to researchers in Oklahoma. The discovery proteomics experiments (Aim 1) will use data-dependent and data independent acquisition (DDA and DIA, respectively) to identify and quantify with label free methods the abundance of several thousand proteins in complex samples. The targeted quantitative proteomics experiments (Aim 2) will use selected reaction monitoring (SRM), parallel reaction monitoring (PRM), and DIA to selectively detect and quantify proteins based on peptides formed by trypsin digestion. To ensure state-wide awareness of the coreâs capabilities and access to these methods, Aim 3 will, a) continue a series of workshops to describe our methods and educate potential users and b) provide vouchers to defray any charges associated with pilot projects.

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