CANCAN - Spatially resolved metabolic measurements in cells and organs during cancer cachexia

Northwestern University At Chicago, Evanston IL

Investigators

Abstract

Cancer cachexia is a multifaceted syndrome characterized by significant loss of body weight and muscle mass, which is not reversible through conventional nutritional support. This condition severely affects the quality of life and survival rates of cancer patients. Understanding the metabolic disruptions underlying cachexia is crucial for developing effective treatments. Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (MALDI-IMS) is a cutting-edge technology that combines the specificity of mass spectrometry with spatial mapping capabilities to visualize the distribution of metabolites and therapeutics directly within tissue sections. This approach enables true metabolic measurements (‘molecular pathology’) at the single-cell level. Importantly, this is a non-destructive method that allows for orthogonal assays to be performed on the same slide, facilitating comprehensive ‘multi-omics’ analyses. By utilizing MALDI-IMS, we aim to examine the systemic metabolic disruptions in various tissues such as muscle, fat, and liver caused by tumors during the progression of cachexia. This technology will enable us to perform spatial metabolomics and spatial metabolic flux analysis, providing detailed insights into how metabolism is altered in specific cell populations within these tissues.