Structure-Function Studies of the Human Cytochrome P450 27 Family

Appalachian State University, Boone NC

Investigators

Abstract

Summary The PI is requesting an administrative supplement to fund the purchase of fast protein liquid chromatography (FPLC) equipment to aid protein purification, specifically an ÄKTA Go from Cytiva Life Sciences. The instrument will be used to upscale protein purification throughput to match recent lab advances in recombinant protein production and enable high-resolution, high-capacity size exclusion chromatography techniques. All experiments in the original parent award (1R35GM147276) utilize recombinantly produced and purified isolated protein, so access to the ÄKTA Go FPLC will enhance all aspects of the parent award.